Assembly, tuning and use of an apertureless near field infrared microscope for protein imaging.

Journal of Visualized Experiments : JoVE
Melissa PauliteGilbert C Walker

Abstract

This paper aims to instruct the reader in the assembly and operation of an infrared near-field microscope for imaging beyond the diffraction limit. The apertureless near-field microscope is a light scattering-type instrument that provides infrared spectra at circa 20 nm resolution. A complete list of components and a step-by-step protocol for use is provided. Common errors in assembly and instrument tuning are discussed. A representative data set that shows the secondary structure of an amyloid fibril is presented.

References

Nov 7, 1999·The Journal of Biological Chemistry·D S YangP E Fraser
Apr 20, 2005·Langmuir : the ACS Journal of Surfaces and Colloids·Pamela Y Meadows, Gilbert C Walker

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Citations

Jul 27, 2011·Journal of Visualized Experiments : JoVE·Patrick J M MurphyJohn Goertz

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