Assessing and influencing the fractional contribution of erythrocyte-bound 59Fe to individual 59Fe tissue content in murine 59Fe distribution studies

Toxicology
Bernadett SzegnerK Schümann

Abstract

Murine proteins of iron homoeostasis are frequently manipulated to investigate the mechanisms of iron-distribution and their toxicological consequences. Beyond subtracting erythrocyte-bound 59Fe of the residual blood content determined for each tissue (subtraction method), procedures are needed to determine 59Fe distribution in murine models of, e.g. inflammation or diabetes that cause local hyperaemia and changes in microcirculation. Two new methods were developed to correct total 59Fe tissue content individually for erythrocyte-bound 59Fe-labelled haem iron. Iron-deficiency and iron-overload was induced in male C57BL6 mice by feeding of respective diets. Distribution of 59Fe between different tissues was determined 24h, 14, and 28 days after intravenous injection of 59Fe trace amounts. Haem-bound 59Fe was separated from non-haem 59Fe in homogenates from all tissues by dispersion in a mix of lipophilic cyclohexanone and hydrophilic H3PO4 (separation method). Moreover, the reduction of 59Fe-labelled tissue blood content was determined in all organs after in vivo saline perfusion via the left ventricle (perfusion method). 59Fe-labelled non-haem iron determined by the separation method was not significantly different from values ...Continue Reading

References

Mar 14, 1998·Proceedings of the National Academy of Sciences of the United States of America·M D FlemingN C Andrews
Jun 26, 2001·Annals of Nutrition & Metabolism·K Schümann
Nov 1, 2002·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Andreas HoeflichEckhard Wolf
Oct 1, 1959·Biochimica Et Biophysica Acta·F W TEALE
Jan 1, 1960·British Journal of Haematology·W D NOYESC A FINCH
Apr 28, 2004·Cell·Matthias W HentzeNancy C Andrews
Mar 11, 2005·The New England Journal of Medicine·Guenter Weiss, Lawrence T Goodnough
Aug 3, 2005·The Journal of Clinical Investigation·Franklin W HuangNancy C Andrews
Feb 1, 2007·Blood·Maja Vujic SpasicMartina U Muckenthaler
Aug 19, 2007·Journal of Trace Elements in Medicine and Biology : Organ of the Society for Minerals and Trace Elements (GMS)·Klaus SchümannNoel W Solomons

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Citations

Oct 22, 2013·Experimental Hematology·Martin KistlerKlaus Schümann

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