Assessing Anti-HCMV Cell Mediated Immune Responses in Transplant Recipients and Healthy Controls Using a Novel Functional Assay.

Frontiers in Cellular and Infection Microbiology
Charlotte J HouldcroftMark R Wills

Abstract

HCMV infection, reinfection or reactivation occurs in 60% of untreated solid organ transplant (SOT) recipients. Current clinical approaches to HCMV management include pre-emptive and prophylactic antiviral treatment strategies. The introduction of immune monitoring to better stratify patients at risk of viraemia and HCMV mediated disease could improve clinical management. Current approaches quantify T cell IFNγ responses specific for predominantly IE and pp65 proteins ex vivo, as a proxy for functional control of HCMV in vivo. However, these approaches have only a limited predictive ability. We measured the IFNγ T cell responses to an expanded panel of overlapping peptide pools specific for immunodominant HCMV proteins IE1/2, pp65, pp71, gB, UL144, and US3 in a cohort of D+R- kidney transplant recipients in a longitudinal analysis. Even with this increased antigen diversity, the results show that while all patients had detectable T cell responses, this did not correlate with control of HCMV replication in some. We wished to develop an assay that could directly measure anti-HCMV cell-mediated immunity. We evaluated three approaches, stimulation of PBMC with (i) whole HCMV lysate or (ii) a defined panel of immunodominant HCMV pep...Continue Reading

References

Nov 8, 2001·Trends in Immunology·M A CooperM A Caligiuri
Nov 5, 2002·Nature Reviews. Immunology·Matthias J Reddehase
Dec 17, 2002·Clinical Immunology : the Official Journal of the Clinical Immunology Society·William H CarrPeter Parham
Apr 2, 2003·Journal of Immunological Methods·Annette Trickett, Yiu Lam Kwan
Sep 10, 2003·The Journal of Infectious Diseases·Maria C VillacresDon J Diamond
Aug 13, 2004·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Alain CoaquetteGeorges Herbein
Mar 30, 2005·The Journal of Experimental Medicine·Torsten BundeFlorian Kern
Apr 12, 2005·American Journal of Health-system Pharmacy : AJHP : Official Journal of the American Society of Health-System Pharmacists·Raymund R Razonable
Sep 9, 2005·The Journal of Experimental Medicine·Andrew W SylwesterLouis J Picker
Nov 23, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Mark R WillsJ G Patrick Sissons
Aug 8, 2006·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·G GernaT Rampino
Jan 6, 2007·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Christian SinzgerGerhard Jahn
Apr 28, 2007·Transplant Infectious Disease : an Official Journal of the Transplantation Society·S WalkerR Khanna
Nov 27, 2008·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·K HadayaJ Villard
Feb 26, 2009·BioTechniques·Richard J StantonGavin W G Wilkinson
Jun 23, 2009·The Journal of Immunology : Official Journal of the American Association of Immunologists·Stéphanie GrasDominique Housset
Dec 3, 2009·Transplant International : Official Journal of the European Society for Organ Transplantation·Albert J EidRaymund R Razonable
Aug 4, 2010·The Journal of Clinical Investigation·Richard J StantonGavin W G Wilkinson
Dec 22, 2010·Expert Review of Molecular Diagnostics·Stefano Giulieri, Oriol Manuel
May 19, 2011·Transplant Infectious Disease : an Official Journal of the Transplantation Society·S BorchersE Mischak-Weissinger
Oct 18, 2011·Current Opinion in Infectious Diseases·Morgan Hakki, Sunwen Chou
Nov 19, 2011·Transplant Infectious Disease : an Official Journal of the Transplantation Society·L F LisboaA Humar
Dec 31, 2011·Blood·Ester B M RemmerswaalIneke J M Ten Berge
May 19, 2012·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·S F AtabaniP D Griffiths
May 23, 2012·Expert Review of Clinical Immunology·Richard R WatkinsRaymund R Razonable
Mar 8, 2013·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·R R RazonableUNKNOWN AST Infectious Diseases Community of Practice
Apr 18, 2013·PLoS Pathogens·Johan SiewieraNabila Jabrane-Ferrat
Apr 27, 2013·International Reviews of Immunology·Leonardo M R Ferreira
Jul 31, 2013·Biology of Blood and Marrow Transplantation : Journal of the American Society for Blood and Marrow Transplantation·Cinja SukdolakBritta Eiz-Vesper

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Citations

Feb 16, 2021·The Journal of Clinical Investigation·Virginia-Maria VlahavaRichard J Stanton

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Methods Mentioned

BETA
flow cytometry
ELISA
PCR
biopsy
density gradient centrifugation
enzyme-linked immunosorbent assay
FCS

Software Mentioned

EliSpot
Spot
FluoroSpot
GraphPad Prism
AID EliSpot
Geneart

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