Assessing biological and technological variability in protein levels measured in pre-diagnostic plasma samples of women with breast cancer

Biomarker Research
Christine Y YehSharon J Pitteri

Abstract

Quantitative proteomics allows for the discovery and functional investigation of blood-based pre-diagnostic biomarkers for early cancer detection. However, a major limitation of proteomic investigations in biomarker studies remains the biological and technical variability in the analysis of complex clinical samples. Moreover, unlike 'omics analogues such as genomics and transcriptomics, proteomics has yet to achieve reproducibility and long-term stability on a unified technological platform. Few studies have thoroughly investigated protein variability in pre-diagnostic samples of cancer patients across multiple platforms. We obtained ten blood plasma "case" samples collected up to 2 years prior to breast cancer diagnosis. Each case sample was paired with a matched control plasma from a full biological sister without breast cancer. We measured protein levels using both mass-spectrometry and antibody-based technologies to: (1) assess the technical considerations in different protein assays when analyzing limited clinical samples, and (2) evaluate the statistical power of potential diagnostic analytes. Although we found inherent technical variation in the three assays used, we detected protein dependent biological signal from the ...Continue Reading

References

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Citations

Sep 1, 2018·Expert Review of Proteomics·Robert PoppChristoph Borchers
Jan 14, 2021·Biomarkers : Biochemical Indicators of Exposure, Response, and Susceptibility to Chemicals·Mohsin AminKathryn A Whitehead

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Methods Mentioned

BETA
protein assay
immunodepletion
reversed phase chromatography
PCR
Assay
PCA

Software Mentioned

RBM
Myriad
Computational Protein Analysis System
Olink
Q3ProteinParser
PeptideProphet

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