PMID: 8962743Dec 1, 1995Paper

Assessing cellular proliferation: what's worth measuring?

Human & Experimental Toxicology
M R Alison

Abstract

The assessment of cellular proliferation is, in the main, carried out on histological material. Ideally, such methods should be applicable to routinely processed tissues, they should be relatively inexpensive and the results easily quantified and interpreted. A knowledge of what is 'normal' within the tissue from both a temporal and spatial point of view is essential. There is a burgeoning literature on novel markers of cell proliferation, usually these are immunohistochemically based, each method with its own unique set of conditions for optimal tissue processing. Some would argue this is simply a process of 'reinventing the wheel' since mitotic counting is a perfectly adequate indicator of proliferation, but we could justly counter that a labelling index (S-phase or growth fraction) is more likely to produce an accurate assessment when the sample size is small. In tumours, tissue heterogeneity is likely to render any single measurement meaningless. To date, there are no reliable validated immunohistochemical markers of the labelling index in animal tissues which visualise naturally occurring proliferation-associated molecules, though this may change shortly.

References

Jan 1, 1991·Virchows Archiv. A, Pathological Anatomy and Histopathology·M R AlisonG M Murphy
May 1, 1988·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Y HayashiT Hoshino
Oct 1, 1985·The Anatomical Record·G ZajicekY Michaeli
May 24, 1984·Nature·M B MathewsJ I Garrels
Jan 15, 1983·International Journal of Cancer. Journal International Du Cancer·J GerdesH Stein
Nov 1, 1980·Cell and Tissue Kinetics·N A Wright, D R Appleton
Dec 1, 1994·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·M AlisonH Pringle
Jul 1, 1994·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·A R SoamesE B Wheeldon

❮ Previous
Next ❯

Citations

Feb 1, 2000·Journal of Cellular Physiology·T Scholzen, J Gerdes
Mar 20, 2001·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·M H BerntssenA K Lundebye
Oct 27, 2004·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Akihiro HirataMasae Tatematsu
Apr 1, 1996·Human & Experimental Toxicology·C C Boyle
Sep 26, 1997·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·C GerlachJ Gerdes
Nov 19, 2003·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Levan MuskhelishviliEric B Henderson
Apr 24, 2010·Cell Proliferation·T-H YenC-W Yang
Nov 7, 2019·Journal of Animal Physiology and Animal Nutrition·Hedia NasriMoncef Bouzouaia
Sep 17, 2019·Canadian Journal of Kidney Health and Disease·Rosalie E O'HaraSunny Hartwig
Aug 9, 2020·Journal of the American Society of Nephrology : JASN·Sierra S MarableJoo-Seop Park

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.