Assessing effects of pressure on tumor and normal tissue physiology using an automated self-calibrated, pressure-sensing probe for diffuse reflectance spectroscopy

Journal of Biomedical Optics
Gregory M PalmerWalter T Lee

Abstract

Diffuse reflectance spectroscopy (DRS) represents a quantitative, noninvasive, nondestructive means of assessing vascular oxygenation, vascularity, and structural properties. However, it is known that such measurements can be influenced by the effects of pressure, which is a major concern for reproducible and operator-independent assessment of tissues. Second, regular calibration is a necessary component of quantitative DRS to account for factors such as lamp decay and fiber bending. Without a means of reliably controlling for these factors, the accuracy of any such assessments will be reduced, and potentially biased. To address these issues, a self-calibrating, pressure-controlled DRS system is described and applied to both a patient-derived xenograft glioma model, as well as a set of healthy volunteers for assessments of oral mucosal tissues. It was shown that pressure had a significant effect on the derived optical parameters, and that the effects on the optical parameters were magnified with increasing time and pressure levels. These findings indicate that not only is it critical to integrate a pressure sensor into a DRS device, but that it is also important to do so in an automated way to trigger a measurement as soon as p...Continue Reading

References

Mar 11, 2006·Journal of Biomedical Optics·Nena M MarínRebecca Richards-Kortum
May 27, 2008·Nature Reviews. Cancer·Mark W DewhirstBenjamin Moeller
Jun 11, 2008·Optics Express·Yalin Ti, Wei-Chiang Lin
Aug 19, 2008·Optics Letters·Bing YuNirmala Ramanujam
Sep 19, 2015·Seminars in Radiation Oncology·Christopher D WilleyGeorge Yancey Gillespie

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