Assessing European Wheat Sensitivities to Parastagonospora nodorum Necrotrophic Effectors and Fine-Mapping the Snn3-B1 Locus Conferring Sensitivity to the Effector SnTox3

Frontiers in Plant Science
Rowena C DownieJames Cockram

Abstract

Parastagonospora nodorum is a necrotrophic fungal pathogen of wheat (Triticum aestivum L.), one of the world's most important crops. P. nodorum mediates host cell death using proteinaceous necrotrophic effectors, presumably liberating nutrients that allow the infection process to continue. The identification of pathogen effectors has allowed host genetic resistance mechanisms to be separated into their constituent parts. In P. nodorum, three proteinaceous effectors have been cloned: SnToxA, SnTox1, and SnTox3. Here, we survey sensitivity to all three effectors in a panel of 480 European wheat varieties, and fine-map the wheat SnTox3 sensitivity locus Snn3-B1 using genome-wide association scans (GWAS) and an eight-founder wheat multi-parent advanced generation inter-cross (MAGIC) population. Using a Bonferroni corrected P ≤ 0.05 significance threshold, GWAS identified 10 significant markers defining a single locus, Snn3-B1, located on the short arm of chromosome 5B explaining 32% of the phenotypic variation [peak single nucleotide polymorphisms (SNPs), Excalibur_c47452_183 and GENE-3324_338, -log10P = 20.44]. Single marker analysis of SnTox3 sensitivity in the MAGIC population located Snn3-B1 via five significant SNPs, defining ...Continue Reading

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Citations

Jan 10, 2020·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Beatrice CorsiJames Cockram
Apr 14, 2019·Planta·Pramod PrasadSubodh Kumar
Jul 3, 2020·Frontiers in Microbiology·Min LinMorten Lillemo
Oct 14, 2020·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Min LinMorten Lillemo
Sep 29, 2020·Genome Biology and Evolution·Danilo PereiraDaniel Croll
Oct 24, 2020·Journal of Integrative Plant Biology·Qi LiDaolong Dou
Jun 3, 2021·International Journal of Molecular Sciences·Dora LiMichael Francki
Nov 28, 2021·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Laura BouvetJames Cockram
Jan 21, 2022·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Amanda R Peters HaugrudJustin D Faris

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Methods Mentioned

BETA
two-hybrid
Genotyping
PCR
SMA
GTPase
dissection

Software Mentioned

qtl
MAGIC
PolyMarker
R
BLASTn
R R
SAM
MATLAB
Genome Association and Prediction Integrated Tool ( GAPIT )
SNP Viewer

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