Assessing glucose and oxygen diffusion in hydrogels for the rational design of 3D stem cell scaffolds in regenerative medicine

Journal of Tissue Engineering and Regenerative Medicine
L FigueiredoP Weiss

Abstract

Hydrogels are attractive biomaterials for replicating cellular microenvironments, but attention needs to be given to hydrogels diffusion properties. A large body of literature shows the promise of hydrogels as 3D culture models, cell expansion systems, cell delivery vehicles, and tissue constructs. Surprisingly, literature seems to have overlooked the important effects of nutrient diffusion on the viability of hydrogel-encapsulated cells. In this paper, we present the methods and results of an investigation into glucose and oxygen diffusion into a silated-hydroxypropylmethylcellulose (Si-HPMC) hydrogel. Using both an implantable glucose sensor and implantable oxygen sensor, we continuously monitored core glucose concentration and oxygen concentration at the centre of hydrogels. We demonstrated that we could tune molecular transport in Si-HPMC hydrogel by changing the polymer concentration. Specifically, the oxygen diffusion coefficient was found to significantly decrease from 3.4 × 10-10 to 2.4 × 10-10  m2  s-1 as the polymer concentration increased from 1% to 4% (w/v). Moreover, it was revealed during in vitro culture of cellularized hydrogels that oxygen depletion occurred before glucose depletion, suggesting oxygen diffusion...Continue Reading

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Citations

Jul 5, 2018·International Journal of Molecular Sciences·Fabien NativelGael Grimandi
Jan 22, 2020·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Anowar H KhanAdam T Melvin
Dec 18, 2020·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Kankan QinFrancisco M Fernandes
Jul 16, 2020·Biotechnology and Bioengineering·Carola SchmitzAntonina Lavrentieva
Mar 5, 2021·Journal of Tissue Engineering and Regenerative Medicine·Kaiwen Zheng, Dajiang Du
Apr 5, 2021·Acta Biomaterialia·Chandralekha ChatterjeeCornelia Lee-Thedieck
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May 4, 2021·Chemical Reviews·Santiago CorreaEric A Appel
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Aug 18, 2018·ACS Applied Materials & Interfaces·Manohar Prasad KoduriFan Gang Tseng
Jul 28, 2020·ACS Applied Bio Materials·Hans PriksPetri-Jaan Lahtvee
Aug 28, 2021·Antioxidants·Jorge OteroIsaac Almendros

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