Assessing loss of imprint methylation in sperm from subfertile men using novel methylation polymerase chain reaction Luminex analysis

Fertility and Sterility
Akiko SatoTakahiro Arima

Abstract

To assess the clinical value of bisulfite polymerase chain reaction Luminex (BPL), an automated, high-throughput procedure for the detection of alterations in DNA methylation. Experimental prospective study. University research laboratory and private in vitro fertilization (IVF) clinic. A total of 337 men, 61 with severe oligozoospermia, 67 with moderate oligozoospermia, and 209 with microscopically normozoospermia. The ejaculated sperm samples after the routine semen analysis with patients' consent. Examination of the methylation patterns of eight imprinted loci in sperm DNA, and confirmation with combined bisulfite PCR restriction analysis (COBRA). A total of 47 cases (13.9%) showed abnormal methylation at one or more imprinted loci (18 paternal, 18 maternal, and 11 cases with alterations of both maternal and paternal imprints). The relative ease of the BPL method provides a practical method within a clinical setting to reduce the likelihood of abnormal samples being used in assisted reproduction treatments.

References

Sep 25, 1997·Human Molecular Genetics·L LefebvreM A Surani
Mar 8, 2002·The New England Journal of Medicine·Michèle HansenSandra Webb
May 23, 2002·American Journal of Human Genetics·Gerald F CoxBernhard Horsthemke
Nov 20, 2002·American Journal of Human Genetics·Michael R DeBaunAndrew P Feinberg
Jan 15, 2003·Journal of Medical Genetics·E R MaherM M Hawkins
Feb 1, 2003·Lancet·Annette C MollFlora E van Leeuwen
Mar 26, 2003·Journal of Microbiological Methods·Sherry A DunbarJames W Jacobson
Mar 10, 2004·Human Reproduction Update·Diana LuciferoJacquetta M Trasler
May 26, 2004·Lancet·Cristina Joana MarquesAlberto Barros
Apr 6, 2005·Human Molecular Genetics·Eamonn R Maher
Dec 13, 2005·Placenta·J McMinnB Tycko
Jan 13, 2006·Human Molecular Genetics·Philippe ArnaudGavin Kelsey
Aug 23, 2006·Human Reproduction·A SatoT Arima
Mar 14, 2007·American Journal of Epidemiology·Sherry L FarrDenise J Jamieson
Jul 20, 2007·Human Molecular Genetics·Hisato KobayashiTakahiro Arima
Oct 9, 2007·Human Reproduction·Sarah BowdinWilliam Reardon
Nov 1, 2007·Archives of Gynecology and Obstetrics·Offer ErezMoshe Mazor
Jan 8, 2008·Molecular Human Reproduction·C J MarquesM Sousa
Jan 17, 2008·Nature Reviews. Genetics·Hiroyuki Sasaki, Yasuhisa Matsui
May 28, 2009·European Journal of Human Genetics : EJHG·Hisato KobayashiTakahiro Arima
Jun 2, 2009·Epigenetics : Official Journal of the DNA Methylation Society·Andreas I DiplasJia Chen

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Citations

Jan 24, 2012·Human Reproduction Update·A P A van MontfoortP de Boer
Dec 25, 2013·Reproductive Biomedicine Online·B C J M FauserUNKNOWN Evian Annual Reproduction (EVAR) Workshop Group 2011
Jun 14, 2014·Asian Journal of Andrology·Ruth Kläver, Jörg Gromoll
Oct 10, 2014·Reproductive Medicine and Biology·Hitoshi HiuraTakahiro Arima
Aug 22, 2013·Expert Opinion on Drug Metabolism & Toxicology·Sarah CampionFederico Goodsaid
Nov 5, 2013·Fertility and Sterility·Edward DereKim Boekelheide
May 30, 2013·Fertility and Sterility·Céline Chalas BoissonnasHélène Jammes
Jan 28, 2015·Epigenetics : Official Journal of the DNA Methylation Society·Juliane KuhtzThomas Haaf
Sep 12, 2015·Fertility and Sterility·Kenneth I AstonDouglas T Carrell
Jul 28, 2015·Congenital Anomalies·Akane KitamuraTakahiro Arima
Jan 14, 2012·Systems Biology in Reproductive Medicine·Ester Anton, Stephen A Krawetz
May 1, 2015·Reproductive Biology and Endocrinology : RB&E·Rakesh SharmaRola F Turki
Dec 6, 2014·Frontiers in Public Health·María Jesús Sánchez-CalabuigPablo Bermejo-Álvarez
Feb 18, 2016·Reproduction : the Official Journal of the Society for the Study of Fertility·Sandra LaurentinoJörg Gromoll
Aug 12, 2016·Clinica Chimica Acta; International Journal of Clinical Chemistry·C ParisiE S Lianidou
Jul 29, 2016·Toxicological Sciences : an Official Journal of the Society of Toxicology·Edward DereKim Boekelheide
Jan 11, 2014·Reproductive Sciences·Xiaoyun ShiYaqin Wu
Sep 8, 2012·Reproduction : the Official Journal of the Society for the Study of Fertility·Michelle M Denomme, Mellissa R W Mann
Aug 14, 2020·Human Reproduction Update·Fredrika ÅseniusSarah J Marzi
Oct 21, 2018·Clinical Epigenetics·Céline BrunoPatricia Fauque
Feb 12, 2017·Scientific Reports·Norio KobayashiTakahiro Arima
Dec 28, 2018·The World Journal of Men's Health·Filippo GiaconeSandro La Vignera
Mar 23, 2021·Fertility and Sterility·Michelle M DenommeMandy G Katz-Jaffe
Jun 4, 2021·Systems Biology in Reproductive Medicine·Rossella CannarellaAldo E Calogero

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