Assessing mechanisms of toxicant response in the amphipod Melita plumulosa through transcriptomic profiling

Aquatic Toxicology
Sharon E HookStuart L Simpson

Abstract

This study describes the function of transcripts with altered abundance in the epibenthic amphipod, Melita plumulosa, following whole-sediment exposure to a series of common environmental contaminants. M. plumulosa were exposed for 48 h to sediments spiked and equilibrated with the following contaminants at concentrations predicted to cause sublethal effects to reproduction: porewater ammonia 30 mg L(-1); bifenthrin at 100 μg kg(-1); fipronil at 50 μg kg(-1); 0.6% diesel; 0.3% crude oil; 250 mg Cu kg(-1); 400 mg Ni kg(-1); and 400 mg Zn kg(-1). RNA was extracted and hybridized against a custom Agilent microarray developed for this species. Although the microarray represented a partial transcriptome and not all features on the array could be annotated, unique transcriptomic profiles were generated for each of the contaminant exposures. Hierarchical clustering grouped the expression profiles together by contaminant class, with copper and zinc, the petroleum products and nickel, and the pesticides each forming a distinct cluster. Many of the transcriptional changes observed were consistent with patterns previously described in other crustaceans. The changes in the transcriptome demonstrated that contaminant exposure caused changes...Continue Reading

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Citations

Feb 28, 2014·Integrated Environmental Assessment and Management·Sharon E HookGraeme E Batley
Jul 6, 2014·Environmental Science and Pollution Research International·Sharon E HookStuart L Simpson
May 12, 2017·Environmental Science & Technology·Katherine J JeppeVincent Pettigrove
Oct 9, 2014·Molecular & Cellular Proteomics : MCP·Judith TrappJean Armengaud
Oct 27, 2017·Environmental Toxicology and Chemistry·Kay T HoRobert M Burgess
Mar 25, 2017·International Journal of Environmental Research and Public Health·David J BealeEnzo A Palombo
Apr 9, 2021·Journal of Environmental Management·Abhinav Dhaka, Pradipta Chattopadhyay
Oct 23, 2019·Environmental Science & Technology·Yick Hang KwanJin Sun

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