Assessing nitrate metabolism in the intestinal tract by measuring breath nitric oxide and nitrous oxide, and its clinical significance

Clinica Chimica Acta; International Journal of Clinical Chemistry
Takahiro Mitsui, Takaharu Kondo

Abstract

The toxicity of dietary nitrate (NO3-) is controversial. One reason is nitrate metabolism in the intestine is so complicated that it is far from fully understood. There is no study measuring breath nitric oxide (NO) and nitrous oxide (N2O) after ingesting vegetables and high-nitrate food at the same time. Breath samples from 10 healthy young and 10 healthy old subjects were collected at 15-min intervals for 5 h after ingestion of 100 g of lettuce and during fasting (control). Breath NO and N2O were analyzed by a chemiluminescence and an IR-PAS analyzer respectively. N2O maximum concentration and excretions increased significantly after ingesting lettuce in each group [303 (30) vs. 750 (81) ppb, 771 (72) vs. 1668 (146) microg in young; 442 (52) vs. 1092 (109) ppb, 1088 (125) vs. 2100 (183) microg in old subjects; mean (SE), P<0.01], while NO did not. In addition, breath NO was strongly influenced by ambient NO, which varied greatly. N2O maximum level in old subjects after ingesting lettuce was higher than that of young subjects (750 vs. 1092 ppb, P<0.05), and significantly higher N2O concentration levels were seen at 30, 45, 60, and 105 min in old subjects. A large amount of N2O produced in the intestine and normal nitrate intak...Continue Reading

References

Dec 1, 1976·Food and Cosmetics Toxicology·S R TannenbaumD Fett
Feb 21, 1985·Nature·D FormanR Doll
Apr 19, 1973·The New England Journal of Medicine·J P KeatingG E Smith
Nov 1, 1982·The American Journal of Clinical Nutrition·M L Burr, P M Sweetnam
Apr 3, 1981·Science·L C GreenP Goldman
Feb 1, 1995·European Journal of Epidemiology·M M Morales-Suárez-VarelaM L Tejerizo-Perez
Jan 10, 1995·Archives of Biochemistry and Biophysics·L BrunelliJ S Beckman
Jan 15, 1994·Lancet·S A KharitonovP J Barnes
Nov 1, 1994·Carcinogenesis·B D VuO G Ekindjian
Apr 7, 1994·Nature·N BenjaminH McKenzie
Jan 1, 1993·Archives of Microbiology·W G Zumft
Jul 1, 1993·European Journal of Cancer Prevention : the Official Journal of the European Cancer Prevention Organisation (ECP)·G P XuP I Reed
Feb 1, 1996·American Journal of Respiratory and Critical Care Medicine·B KimberlyW E Holden
Dec 1, 1996·The European Respiratory Journal·J O LundbergK Alving
Feb 1, 1997·Gut·P Vallance
Jul 2, 1999·Digestive Diseases and Sciences·T Mitsui, T Kondo
Jan 1, 2000·The British Journal of Nutrition·G M McKnightM H Golden
May 2, 2000·Food Additives and Contaminants·G YsartN Harrison
Jul 21, 2000·Environmental Health Perspectives·L KnobelochH Anderson
Feb 24, 2001·Respiratory Medicine·A C OlinK Torén

❮ Previous
Next ❯

Citations

Jun 15, 2004·Clinica Chimica Acta; International Journal of Clinical Chemistry·Takahiro Mitsui, Takaharu Kondo
Jan 10, 2018·Wiley Interdisciplinary Reviews. Systems Biology and Medicine·D Rose Ewald, Susan C J Sumner
Nov 11, 2011·Journal of Breath Research·Agnieszka UlanowskaBogusław Buszewski

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.