Assessing Performance of Spore Samplers in Monitoring Aeromycobiota and Fungal Plant Pathogen Diversity in Canada

Applied and Environmental Microbiology
Wen ChenC André Lévesque

Abstract

Spore samplers are widely used in pathogen surveillance but not so much for monitoring the composition of aeromycobiota. In Canada, a nationwide spore-sampling network (AeroNet) was established as a pilot project to assess fungal community composition in air and rain samples collected using three different spore samplers in the summers of 2010 and 2011. Metabarcodes of the internal transcribed spacer (ITS) were exhaustively characterized for three of the network sites, in British Columbia (BC), Québec (QC), and Prince Edward Island (PEI), to compare performance of the samplers. Sampler type accounted for ca. 20% of the total explainable variance in aeromycobiota compositional heterogeneity, with air samplers recovering more Ascomycota and rain samplers recovering more Basidiomycota. Spore samplers showed different abilities to collect 27 fungal genera that are plant pathogens. For instance, Cladosporium spp., Drechslera spp., and Entyloma spp. were collected mainly by air samplers, while Fusarium spp., Microdochium spp., and Ustilago spp. were recovered more frequently with rain samplers. The diversity and abundance of some fungi were significantly affected by sampling location and time (e.g., Alternaria and Bipolaris) and weat...Continue Reading

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Citations

Sep 28, 2018·Current Allergy and Asthma Reports·Brett J Green
May 2, 2020·FEMS Microbiology Ecology·Miguel A RedondoJonàs Oliva
Feb 2, 2020·Applied Microbiology and Biotechnology·Nicola LuchiAlberto Santini

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Datasets Mentioned

BETA
SRP095430
SRS1872326
SRS1872422

Methods Mentioned

BETA
PCR

Software Mentioned

FUNGuild
RAM
vegan
R package nlme
lattice
R package MASS
corrplot
HierDiversity
R
R package vcd

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