Assessing routes of hepatitis C transmission in HIV-infected men who have sex with men using single genome sequencing

PloS One
Hui LiDaniel S Fierer

Abstract

The epidemic of hepatitis C virus (HCV) infection among HIV-infected men who have sex with men (MSM) is in its second decade, but the routes of transmission remain poorly understood. We hypothesized that by pairing single genome sequencing (SGS), to enumerate infecting HCV genomes (viruses), with detailed sexual and drug histories, we could gain insight into the routes of transmission among MSM. We used SGS to analyze blood specimens from eight HIV-infected MSM who had 10 episodes of acute (seronegative) or early HCV infections. Seven of eight men reported condomless receptive anal intercourse (CRAI), six with rectal exposure to semen, and all eight denied rectal trauma or bleeding. Of the 10 HCV infections, eight resulted from transmission of a single virus; one infection resulted from transmission of either one or a few (three or four) closely-related viruses; and one infection resulted from transmission of >10 distinct viruses. The participant infected by >10 viruses reported sharing injection equipment for methamphetamine during sex. Two other participants also injected methamphetamine during sex but they did not share injection equipment and were infected by a single virus. Conclusions: Most HCV infections of HIV-infected ...Continue Reading

References

Jun 27, 1996·The New England Journal of Medicine·C Conry-CantilenaJ W Shih
Oct 8, 2003·Systematic Biology·Stéphane Guindon, Olivier Gascuel
Nov 23, 2006·Nature Medicine·Jason M BrenchleyDaniel C Douek
May 21, 2008·Proceedings of the National Academy of Sciences of the United States of America·Brandon F KeeleGeorge M Shaw
Oct 2, 2008·HIV Medicine·B G GazzardUNKNOWN BHIVA Treatment Guidelines Writing Group
Feb 6, 2009·Journal of Virology·M-R AbrahamsUNKNOWN Center for HIV-AIDS Vaccine Immunology Consortium
Mar 30, 2010·Journal of Forensic Sciences·Robert G Weston
Apr 9, 2010·Journal of Virology·Katharine J BarGeorge M Shaw
May 21, 2010·PLoS Pathogens·Hui LiGeorge M Shaw
Aug 16, 2012·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Gilles WandelerUNKNOWN Swiss HIV Cohort Study
Mar 28, 2013·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Mallory D WittChloe L Thio
Dec 18, 2013·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Daniel S FiererUNKNOWN New York Acute Hepatitis C Surveillance Network
Jun 20, 2014·The Journal of Infectious Diseases·Roger D KouyosUNKNOWN Swiss HIV Cohort Study (SHCS)
Feb 20, 2015·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Jonathan E VolkC Bradley Hare
Mar 13, 2015·Journal of Acquired Immune Deficiency Syndromes : JAIDS·Ludwig ApersAnne Buvé
Jul 15, 2015·Current HIV/AIDS Reports·Emma Kaplan-Lewis, Daniel Seth Fierer
May 18, 2016·Open Forum Infectious Diseases·Samuel S TurnerDaniel S Fierer
Dec 26, 2016·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Andrew L FosterDaniel S Fierer
May 15, 2018·Frontiers in Immunology·Muriel LavieJean Dubuisson
Jul 1, 2018·Liver International : Official Journal of the International Association for the Study of the Liver·Laurent CotteUNKNOWN Dat'AIDS study Group
Mar 12, 2019·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Arunasingam AbayasingamUNKNOWN InC3 Study Group
May 22, 2019·The Journal of Clinical Investigation·Muhammad N ZahidKatharine J Bar
Sep 29, 2019·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·David ChromyFlorian Mayer

❮ Previous
Next ❯

Datasets Mentioned

BETA
MT416764
MT417181

Methods Mentioned

BETA
glycosylation

Software Mentioned

Geneious Prime
Highlighter
PhyML

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

© 2021 Meta ULC. All rights reserved