Abstract
Given the importance of β-adrenoceptor signalling in regulating cardiac structure and function, robust protocols are required to assess potential alterations in such regulation in murine models in vivo. Echocardiography was performed in naïve and stressed (isoprenaline; 30μg/g/day s.c. for up to 14days) mice, in the absence or presence of acute β-adrenergic stimulation (dobutamine 0.75μg/g, i.p.). Controls received saline infusion and/or injection. Hearts were additionally analysed gravimetrically, histologically and biochemically. In naïve mice, acute β-adrenoceptor stimulation with dobutamine increased heart rate, left ventricular (LV) fractional shortening (LVFS), ejection fraction (LVEF) and wall thickness and decreased LV diameter (p<0.05). In stressed mice, dobutamine failed to induce further inotropic and chronotropic responses. Furthermore, following dobutamine injection, these mice exhibited lower LVEF and LVFS at identical heart rates, relative to corresponding controls. Sustained isoprenaline infusion induced LV hypertrophy (increased heart weight, heart weight/body weight ratio, heart weight/tibia length ratio and LV wall thickness (p<0.05)) by 3days, with little further change at 14days. In contrast, increases in L...Continue Reading
References
Jul 1, 1988·Circulation Research·P ButtrickJ Scheuer
Oct 11, 1994·Proceedings of the National Academy of Sciences of the United States of America·C A MilanoR J Lefkowitz
Nov 5, 1997·Journal of Molecular and Cellular Cardiology·R K KudejS F Vatner
Apr 3, 2001·Circulation Research·F WiesmannS Neubauer
Oct 13, 2001·Journal of Magnetic Resonance Imaging : JMRI·S P WilliamsN van Bruggen
Apr 6, 2002·Circulation Research·YeQing PiJeffery W Walker
Jun 5, 2003·The Journal of Clinical Investigation·Timothy D O'ConnellPaul C Simpson
Mar 16, 2004·American Journal of Physiology. Heart and Circulatory Physiology·Yutaka KitagawaMiyako Takaki
Feb 3, 2009·Perfusion·M AndersonDf Larson
Oct 3, 2009·Nature Protocols·Eiji KinoshitaTohru Koike
Mar 20, 2010·Cardiovascular Research·Ian G WebbMichael S Marber
Jul 6, 2010·Journal of Molecular and Cellular Cardiology·Weihua SongSteven Marston
Dec 23, 2011·Basic Research in Cardiology·Hendrik L BooysenAngela J Woodiwiss
Nov 6, 2012·Journal of Magnetic Resonance Imaging : JMRI·Urszula TyrankiewiczStefan Chlopicki
Apr 17, 2013·Journal of Molecular and Cellular Cardiology·Tong TangH Kirk Hammond
Aug 8, 2013·Journal of Translational Medicine·Hailong DongTakeshi Tsuda
Nov 28, 2013·The Journal of Biological Chemistry·Alexandra J CandasamyMetin Avkiran
Mar 20, 2015·The Journal of Pharmacology and Experimental Therapeutics·Matthew D PuhlJoseph T Coyle
May 24, 2015·American Journal of Physiology. Heart and Circulatory Physiology·Sarah-Lena PuhlChristoph Maack
Jun 17, 2015·Journal of Molecular and Cellular Cardiology·Michael GrimmJoan Heller Brown
Jul 22, 2015·Molecular and Cellular Biology·Guijun YanJianxin Sun
Citations
Dec 25, 2016·American Journal of Physiology. Heart and Circulatory Physiology·Sparkle Russell-PuleriJohn M Tarbell
Sep 12, 2018·Cardiovascular Research·Sarah-Lena PuhlMetin Avkiran
Aug 2, 2017·Pflügers Archiv : European journal of physiology·Esther GiehlBarbara E Ehrlich
Feb 24, 2018·British Journal of Pharmacology·Catherine D E WilderMichael J Curtis
Sep 30, 2020·Disease Models & Mechanisms·Tatianna Wai Ying WongRonald D Cohn
Nov 21, 2019·International Journal of Hyperthermia : the Official Journal of European Society for Hyperthermic Oncology, North American Hyperthermia Group·Anna C CrouchJoan M Greve
Dec 19, 2020·The Journal of Steroid Biochemistry and Molecular Biology·Kimberly StratfordMehdi Hazari
Mar 26, 2021·PeerJ·Elisabeth Walsh-WilkinsonJacques Couet
Jun 2, 2021·Scientific Reports·Anne Marie W BartoschJohn M Tarbell
Jul 15, 2021·Scientific Reports·Sarah-Lena PuhlSabine Steffens
Sep 28, 2021·Frontiers in Physiology·Hannah R LewisMetin Avkiran
Nov 5, 2021·Frontiers in Cell and Developmental Biology·Malgorzata Tokarska-SchlattnerUwe Schlattner