Assessing structural and functional responses of murine hearts to acute and sustained β-adrenergic stimulation in vivo

Journal of Pharmacological and Toxicological Methods
Sarah-Lena PuhlMetin Avkiran

Abstract

Given the importance of β-adrenoceptor signalling in regulating cardiac structure and function, robust protocols are required to assess potential alterations in such regulation in murine models in vivo. Echocardiography was performed in naïve and stressed (isoprenaline; 30μg/g/day s.c. for up to 14days) mice, in the absence or presence of acute β-adrenergic stimulation (dobutamine 0.75μg/g, i.p.). Controls received saline infusion and/or injection. Hearts were additionally analysed gravimetrically, histologically and biochemically. In naïve mice, acute β-adrenoceptor stimulation with dobutamine increased heart rate, left ventricular (LV) fractional shortening (LVFS), ejection fraction (LVEF) and wall thickness and decreased LV diameter (p<0.05). In stressed mice, dobutamine failed to induce further inotropic and chronotropic responses. Furthermore, following dobutamine injection, these mice exhibited lower LVEF and LVFS at identical heart rates, relative to corresponding controls. Sustained isoprenaline infusion induced LV hypertrophy (increased heart weight, heart weight/body weight ratio, heart weight/tibia length ratio and LV wall thickness (p<0.05)) by 3days, with little further change at 14days. In contrast, increases in L...Continue Reading

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Citations

Dec 25, 2016·American Journal of Physiology. Heart and Circulatory Physiology·Sparkle Russell-PuleriJohn M Tarbell
Aug 2, 2017·Pflügers Archiv : European journal of physiology·Esther GiehlBarbara E Ehrlich
Nov 21, 2019·International Journal of Hyperthermia : the Official Journal of European Society for Hyperthermic Oncology, North American Hyperthermia Group·Anna C CrouchJoan M Greve
Dec 19, 2020·The Journal of Steroid Biochemistry and Molecular Biology·Kimberly StratfordMehdi Hazari
Jul 15, 2021·Scientific Reports·Sarah-Lena PuhlSabine Steffens

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