Assessing the bipotency of in vitro-derived neuromesodermal progenitors

Anestis Tsakiridis, Valerie Wilson


Retrospective clonal analysis in the mouse has demonstrated that the posterior spinal cord neurectoderm and paraxial mesoderm share a common bipotent progenitor. These neuromesodermal progenitors (NMPs) are the source of new axial structures during embryonic rostrocaudal axis elongation and are marked by the simultaneous co-expression of the transcription factors T(Brachyury) (T(Bra)) and Sox2. NMP-like cells have recently been derived from pluripotent stem cells in vitro following combined stimulation of Wnt and fibroblast growth factor (FGF) signaling. Under these conditions the majority of cultures consist of T(Bra)/Sox2 co-expressing cells after 48-72 hours of differentiation. Although the capacity of these cells to generate posterior neural and paraxial mesoderm derivatives has been demonstrated at the population level, it is unknown whether a single in vitro-derived NMP can give rise to both neural and mesodermal cells. Here we demonstrate that T(Bra) positive cells obtained from mouse epiblast stem cells (EpiSCs) after culture in NMP-inducing conditions can generate both neural and mesodermal clones. This finding suggests that, similar to their embryonic counterparts, in vitro-derived NMPs are truly bipotent and can thus...Continue Reading


May 3, 2005·Genesis : the Journal of Genetics and Development·Phillip H WhiteDeborah L Chapman
Sep 6, 2005·Development·Mariana Delfino-MachínKate G Storey
Jun 29, 2007·Nature·I Gabrielle M BronsLudovic Vallier
Jul 6, 2007·Development·Noemí Cambray, Valerie Wilson
Apr 28, 2009·Development·Valerie WilsonKate G Storey
Sep 18, 2009·Developmental Cell·Elena TzouanacouJean-François Nicolas


Dec 23, 2017·International Journal of Molecular Sciences·Jing ZhouPatricia Murray
Aug 11, 2018·ELife·Thomas Jr FrithAnestis Tsakiridis
Sep 28, 2019·Cellular and Molecular Life Sciences : CMLS·Moisés Mallo
Mar 8, 2016·ELife·Aida Rodrigo Albors, Kate G Storey
Feb 18, 2021·Development·Filip J WymeerschAnestis Tsakiridis
Apr 3, 2021·Cellular and Molecular Life Sciences : CMLS·Karolina PunovuoriSally Lowell
Apr 21, 2021·Developmental Cell·Elena Gonzalez-GobarttElisa Martí
Sep 4, 2015·Development·Domingos HenriqueKate G Storey
May 14, 2021·Frontiers in Cellular Neuroscience·Zachary T Olmsted, Janet L Paluh

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