Assessing the cytotoxic potential of glycoalkaloidic extract in nanoparticles against bladder cancer cells

The Journal of Pharmacy and Pharmacology
Mariza MirandaMaria V L B Bentley

Abstract

This study proposed to use the nanotechnology to deliver glycoalkaloidic extract (AE) to bladder cancer cells, evaluating their activity in 2D and 3D models and the biological mechanism of cell death. NPs were prepared by nanoprecipitation method using polylactic acid (PLA) and characterized considering their size, charge, particle concentration and stability. The cytotoxicity was evaluated in 2D and 3D model, and the apoptosis and cell cycle were investigated using flow cytometry. NPs loading AE (NP-AE) had diameter around 125 ± 6 nm (PdI <0.1) and negative charge. The encapsulation efficiency of SM and SS was higher than 85% for both compounds. The obtained formulation showed a significant in-vitro cytotoxic effect against RT4 cells in a dose-dependent manner with IC50 two fold lower than the free AE. The cytotoxic effect of NP-AE was mediated by apoptosis and cell cycle arrested in the S phase. RT4 cells cultured under 3D conditions exhibited a higher resistance to the treatments (IC50 ~ three fold higher than in 2D cell culture). The NP-AE might be a promising nanocarrier to load and deliver glycoalkaloids against bladder cancer.

References

Sep 1, 2004·Biochemical and Biophysical Research Communications·Chia-Hua LiangKou-Wha Kuo
May 3, 2007·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Alastair H KyleAndrew I Minchinton
Jul 10, 2007·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·L Y ShiuK W Kuo
Nov 21, 2007·Journal of Ethnopharmacology·Y B JiX Zou
Mar 6, 2009·Small·Jungwoo LeeNicholas A Kotov
Jul 8, 2009·Actas urologicas españolas·Marko BabjukUNKNOWN Asociación Europea de Urología
Feb 24, 2010·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·K K BhutaniS Linder
Mar 1, 2011·Journal of Bioscience and Bioengineering·Ayumi OshikataRyuichi Ueoka
Sep 8, 2011·Langmuir : the ACS Journal of Surfaces and Colloids·R StanimirovaE Pelan
Dec 17, 2011·Journal of Ethnopharmacology·Xia DingSi-Guo Gao
Jan 28, 2012·Parasitology Research·Mariza Abreu MirandaJairo Kenupp Bastos
Feb 10, 2012·Journal of Natural Products·David J Newman, Gordon M Cragg
Oct 17, 2012·World Journal of Urology·Oscar Rodriguez FabaH Villavicencio
May 17, 2014·Assay and Drug Development Technologies·Rasheena EdmondsonLiju Yang
Jan 1, 2014·Clinical and Translational Medicine·Mounira El DemeryPierre-Jean Lamy
Jul 15, 2015·Oncology Letters·Xiaodong XieGenbao Shao
Oct 3, 2015·Current Pharmaceutical Design·Krzysztof Wrzesinski, Stephen J Fey
Aug 16, 2016·Colloids and Surfaces. B, Biointerfaces·Siram KarthikGregory Franklin
Jan 6, 2017·CA: a Cancer Journal for Clinicians·Rebecca L SiegelAhmedin Jemal
Jan 7, 2017·SICOT-J·Saroj RaiHong Wang
Mar 24, 2017·Colloids and Surfaces. B, Biointerfaces·Boris Rodenak-KladniewGuillermo R Castro
Apr 21, 2017·Cancer Nursing·Mariska C M van der LansAnnoek E C Broers
Sep 4, 2017·Materials Science & Engineering. C, Materials for Biological Applications·Carina I C Crucho, Maria Teresa Barros
Dec 31, 2017·Biochimica Et Biophysica Acta. General Subjects·Greta JarockyteRicardas Rotomskis

❮ Previous
Next ❯

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis