Assessing the effect of an antimicrobial wound dressing on biofilms

Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society
S L PercivalEmma J Woods

Abstract

To date the effect of silver-containing wound dressings on biofilms, known to be present in chronic wounds, has not been determined or documented. In this current study, we aimed to determine the antimicrobial effect of a silver-containing dressing on biofilms grown in a chambered slide model. Before the addition of a wound dressing onto a 24-hour biofilm, composed of either Pseudomonas aeruginosa, Enterobacter cloacae, Staphylococcus aureus, or a mixed bacterial community, a fluorescent dye was applied. This enabled the viability of sessile bacteria to be monitored in real-time, using a rapid form of confocal laser scanning microscopy over a contact time period of 48 hours. By analyzing all the three-dimensional data generated from the confocal time-lapse sequences, 90% of all sessile bacteria within the biofilm were observed to progressively turn red (i.e., died) within 24 hours. Total bacterial kill in the biofilm was achieved after 48 hours. This research has shown that the dressing was effective in killing the tested bacteria evident in both the tested mono and polymicrobial biofilms, which provides valuable evidence that this dressing may have an effect on biofilms found in recalcitrant chronic wounds.

References

Mar 21, 2001·Antimicrobial Agents and Chemotherapy·K Lewis
Apr 5, 2002·Clinical Microbiology Reviews·Rodney M Donlan, J William Costerton
Jan 28, 2003·Critical Reviews in Microbiology·H LeclercE Dei-Cas
Oct 21, 2004·International Journal of Medical Microbiology : IJMM·Iris FedtkeAndreas Peschel
Apr 21, 2006·The Veterinary Journal·M B MelchiorJ Fink-Gremmels
Aug 10, 2006·Surgical Infections·Gianfranco Donelli

❮ Previous
Next ❯

Citations

Apr 10, 2010·The Journal of Antimicrobial Chemotherapy·Katja E HillDavid W Thomas
Jan 30, 2013·Plastic and Reconstructive Surgery·Akhil K SethSeok J Hong
Dec 23, 2011·Southern Medical Journal·William K JohnjulioChristopher Post
Sep 22, 2010·Antimicrobial Agents and Chemotherapy·Victoria KostenkoRobert John Martinuzzi
Jan 30, 2013·Diabetes, Metabolic Syndrome and Obesity : Targets and Therapy·Crystal HolmesBlaise R Boles
Jul 3, 2013·Clinical Orthopaedics and Related Research·Herrick J SiegelJason Gay
Sep 19, 2014·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Akhil K SethThomas A Mustoe
Aug 28, 2014·Critical Reviews in Microbiology·Steven L PercivalBenjamin A Lipsky
Aug 30, 2012·Critical Reviews in Microbiology·Jean-Yves Maillard, Philippe Hartemann
May 9, 2015·Materials Science & Engineering. C, Materials for Biological Applications·F PaladiniM Pollini
Jul 5, 2011·Burns : Journal of the International Society for Burn Injuries·Merlin GuggenheimBernhard Guggenheim
Dec 7, 2010·Burns : Journal of the International Society for Burn Injuries·Adrienne A HammondAbdul N Hamood
Nov 16, 2010·The Surgical Clinics of North America·Carrie E Black, J William Costerton
Apr 14, 2009·Veterinary Microbiology·E J WoodsS L Percival
Feb 24, 2009·Biomaterials·Evan M HetrickMark H Schoenfisch
Jul 25, 2009·The Journal of Investigative Dermatology·Jo M MartinGerald S Lazarus
Oct 4, 2008·International Journal of Pharmaceutics·Simon GaisfordDavid Parsons
Sep 22, 2012·International Wound Journal·David Leaper
Nov 19, 2011·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Steven L PercivalTyler Okel
Apr 5, 2011·International Wound Journal·Mary Cloud B AmmonsGarth A James
Sep 19, 2012·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Steven L PercivalJohn W Costerton
Nov 17, 2010·International Wound Journal·Charne Nicole MillerNick Santamaria
Feb 21, 2013·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Akhil K SethRobert D Galiano
Oct 7, 2011·International Wound Journal·Steven L PercivalDavid W Williams
Dec 17, 2010·International Wound Journal·Collette H Thomson
Apr 27, 2011·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Steven L PercivalJohn G Thomas
Aug 25, 2016·Plastic and Reconstructive Surgery·Ersilia L AnghelChristopher E Attinger
Jun 14, 2016·The British Journal of Dermatology·G A OkoyeG Lazarus
Jan 22, 2017·Advances in Therapy·George Han, Roger Ceilley
Jun 13, 2009·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·M C PeralJ C Valdez
Mar 12, 2015·British Journal of Community Nursing·Kumal Rajpaul
Jan 14, 2012·Journal of Wound Care·S MalicD W Williams
Oct 20, 2018·Advances in Skin & Wound Care·Anne-Marie Salisbury, Steven L Percival

❮ Previous
Next ❯

Related Concepts

Related Feeds

Biofilm & Infectious Disease

Biofilm formation is a key virulence factor for a wide range of microorganisms that cause chronic infections.Here is the latest research on biofilm and infectious diseases.

Related Papers

Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society
Stephen C DavisPatricia M Mertz
Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society
Garth A JamesPhilip S Stewart
Dermatologic Surgery : Official Publication for American Society for Dermatologic Surgery [et Al.]
Catherine Harrison-BalestraPatricia M Mertz
Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society
Yan SunRandall D Wolcott
Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society
Thomas BjarnsholtMichael Givskov
© 2021 Meta ULC. All rights reserved