Assessing the effects of Cry2Aa protein on Habrobracon hebetor (Hymenoptera: Braconidae), a parasitoid of Indian meal moth, Plodia interpunctella (lepidoptera: Pyralidae)

Ecotoxicology and Environmental Safety
Zhengjie WangHong-Xia Hua

Abstract

Transgenic crops express Cry proteins exhibit high resistant to target insect pests. When we evaluate the effects of Cry proteins on the parasitoid of target insect pest via tritrophic experiments (transgenic plant-target insect pest-parasitoid) host quality of parasitoids might decrease because of insecticidal protein ingestion, this would cause host-quality mediated effects and influence the accuracy of biosafety assessment. In the current study, high dose of Cry2Aa protein was injected into the hemolymph of Plodia interpunctella by microinjection, and the hemolymph was used as the carrier to deliver Cry protein to Habrobracon hebetor, which has been previously reported as an ectoparasitoid of P. interpunctella larval, in order to avoid the "host-quality mediated effects". Results showed that injected Cry2Aa remained at high concentration and bioactive in the hemolymph of P. interpunctella parasitized by H. hebetor, the hemolymph of P. interpunctella could be used as carriers of Cry protein to H. hebetor, and high dose of Cry2Aa have no negative impacts on the development time, weight of pupa, sex ratio, adults weight (male and female), adult longevity and fecundity, and the activity of stress-related enzymes of H. hebetor. H...Continue Reading

References

Apr 18, 1998·Proceedings of the National Academy of Sciences of the United States of America·X ChengI Altosaar
Apr 13, 2000·Annual Review of Entomology·P C Matteson
Sep 28, 2005·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Hao ChenQifa Zhang
Oct 10, 2007·Proceedings of the National Academy of Sciences of the United States of America·Qifa Zhang
Mar 27, 2010·Trends in Microbiology·Ben RaymondNeil Crickmore
Apr 15, 2010·Environmental Entomology·Mukti N Ghimire, Thomas W Phillips
Mar 8, 2011·Insect Biochemistry and Molecular Biology·Alejandra BravoMario Soberón
Jan 13, 2015·Science China. Life Sciences·JiKun HuangZhuRong Huang
Mar 10, 2017·Plant Biotechnology Journal·Yunhe LiJörg Romeis

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Citations

Sep 6, 2020·Ecotoxicology and Environmental Safety·Cong DangGongyin Ye

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