Assessing the impact of denitrifier-produced nitric oxide on other bacteria

Applied and Environmental Microbiology
Peter S ChoiJames P Shapleigh

Abstract

A series of experiments was undertaken to learn more about the impact on other bacteria of nitric oxide (NO) produced during denitrification. The denitrifier Rhodobacter sphaeroides 2.4.3 was chosen as a denitrifier for these experiments. To learn more about NO production by this bacterium, NO levels during denitrification were measured by using differential mass spectrometry. This revealed that NO levels produced during nitrate respiration by this bacterium were in the low muM range. This concentration of NO is higher than that previously measured in denitrifiers, including Achromobacter cycloclastes and Paracoccus denitrificans. Therefore, both 2.4.3 and A. cycloclastes were used in this work to compare the effects of various NO levels on nondenitrifying bacteria. By use of bacterial overlays, it was found that the NO generated by A. cycloclastes and 2.4.3 cells during denitrification inhibited the growth of both Bacillus subtilis and R. sphaeroides 2.4.1 but that R. sphaeroides 2.4.3 caused larger zones of inhibition in the overlays than A. cycloclastes. Both R. sphaeroides 2.4.3 and A. cycloclastes induced the expression of the NO stress response gene hmp in B. subtilis. Taken together, these results indicate that there is ...Continue Reading

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Citations

Jun 7, 2008·Molecular Plant-microbe Interactions : MPMI·Celeste Molina-FaveroLorenzo Lamattina
Feb 13, 2007·Journal of Bacteriology·Annika RogstamClaes von Wachenfeldt
Jul 7, 2009·Annual Review of Microbiology·Peter Zuber
Jan 8, 2013·Bioscience, Biotechnology, and Biochemistry·Hiroyuki AraiSamuel Kaplan
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Oct 1, 2009·Environmental Microbiology Reports·Quan Yuan, Yahai Lu
Nov 23, 2011·Biochemical Society Transactions·Kartik ChandranMark C M van Loosdrecht
Feb 26, 2016·Environmental Microbiology·Constance A RocoJames P Shapleigh
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Dec 17, 2008·Environmental Science & Technology·Richard L Smith, Tadashi Yoshinari
Apr 22, 2018·Environmental Science & Technology·Rathnayake M L D RathnayakeSatoshi Okabe

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