Assessing the Inhibitory Activity of Oligonucleotides on TLR7 Sensing

Methods in Molecular Biology
Jonathan Ferrand, Michael P Gantier

Abstract

Aberrant sensing of self-nucleic acids by Toll-like receptor (TLR) 7, 8, or 9 is associated with several autoimmune disorders, including systemic lupus erythematosus (SLE), rheumatoid arthritis, psoriasis, or systemic sclerosis. In recent years, several classes of synthetic oligonucleotides have been shown to antagonize sensing of immunostimulatory nucleic acids by TLR7/8/9, indicating that these molecules could have therapeutic applications in such autoimmune diseases. Conversely, synthetic oligonucleotides used in therapeutic technologies such as antisense and microRNA inhibitors also have the potential to inhibit TLR7/8/9 sensing, rendering patients more susceptible to viral/bacterial infections. This chapter describes a protocol to define the inhibitory activity of synthetic oligonucleotides on TLR7.

Citations

Dec 22, 2017·RNA·Charlotte NejadMichael P Gantier
Feb 9, 2018·Frontiers in Genetics·Charlotte NejadMichael P Gantier
Apr 5, 2018·Frontiers in Cellular and Infection Microbiology·Jonathan FerrandMichael P Gantier
Jun 1, 2021·Nucleic Acids Research·Roxane ValentinMichael P Gantier

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