Assessing the JEOL CRYO ARM 300 for high-throughput automated single-particle cryo-EM in a multiuser environment

IUCrJ
Marcus FislageRouslan G Efremov

Abstract

Single-particle cryo-EM has become an indispensable structural biology method. It requires regular access to high-resolution electron cryogenic microscopes. To fully utilize the capacity of the expensive high-resolution instruments, the time used for data acquisition and the rate of data collection have to be maximized. This in turn requires high stability and high uptime of the instrument. One of the first 300 kV JEOL CRYO ARM 300 microscopes has been installed at the cryo-EM facility BECM at VIB-VUB, Brussels, where the microscope is used for continuous data collection on multiple projects. Here, the suitability and performance of the microscope is assessed for high-throughput single-particle data collection. In particular, the properties of the illumination system, the stage stability and ice contamination rates are reported. The microscope was benchmarked using mouse heavy-chain apoferritin which was reconstructed to a resolution of 1.9 Å. Finally, uptime and throughput statistics of the instrument accumulated during the first six months of the facility operation in user access mode are reported.

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Citations

May 6, 2021·Acta Crystallographica. Section D, Structural Biology·Rouslan G Efremov, Annelore Stroobants
Oct 5, 2021·Acta Crystallographica. Section D, Structural Biology·Keitaro YamashitaGarib N Murshudov

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Datasets Mentioned

BETA
EMPIAR
EMPIAR-10408

Software Mentioned

SerialEM
ELMO
RELION
JEOL
PHENIX
AlignToItem
MotionCor2
EMPIAR

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