Assessing the properties of internal standards for quantitative matrix-assisted laser desorption/ionization mass spectrometry of small molecules

Rapid Communications in Mass Spectrometry : RCM
Lekha Sleno, Dietrich A Volmer

Abstract

Growing interest in the ability to conduct quantitative assays for small molecules by matrix-assisted laser desorption/ionization (MALDI) has been the driving force for several recent studies. This present work includes the investigation of internal standards for these analyses using a high-repetition rate MALDI triple quadrupole instrument. Certain physicochemical properties are assessed for predicting possible matches for internal standards for different small molecules. The importance of similar molecular weight of an internal standard to its analyte is seen through experiments with a series of acylcarnitines, having a fixed charge site and growing alkyl chain length. Both acetyl- and hexanoyl-carnitine were systematically assessed with several other acylcarnitine compounds as internal standards. The results clearly demonstrate that closely matched molecular weights between analyte and internal standard are essential for acceptable quantitation results. Using alpha-cyano-4-hydroxycinnamic acid as the organic matrix, the similarities between analyte and internal standard remain the most important parameter and not necessarily their even distribution within the solid sample spot. Several 4-quinolone antibiotics as well as a di...Continue Reading

References

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Citations

Jun 6, 2006·Journal of the American Society for Mass Spectrometry·Jay J CorrThomas R Covey
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Jan 29, 2011·Journal of Clinical Research in Pediatric Endocrinology·John W Honour
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Dec 1, 2006·Journal of Mass Spectrometry : JMS
Feb 13, 2007·Rapid Communications in Mass Spectrometry : RCM·David C Grant, Robert J Helleur
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Jun 1, 2013·Rapid Communications in Mass Spectrometry : RCM·Katharina M OstermannDavid C Kasper
Jan 25, 2011·Journal of Mass Spectrometry : JMS·Tiffany PortaGérard Hopfgartner
Dec 10, 2014·Analytical and Bioanalytical Chemistry·Tiffany PortaGérard Hopfgartner
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