Assessing Zika virus replication and the development of Zika-specific antibodies after a mid-gestation viral challenge in guinea pigs

PloS One
Craig J BierleMark R Schleiss

Abstract

Primary Zika virus (ZIKV) infections that occur during pregnancy can cause spontaneous abortion and profoundly disrupt fetal development. While the full range of developmental abnormalities associated with congenital Zika syndrome is not yet known, severe cases of the syndrome can present with microcephaly, extensive neurologic and ocular damage, and pronounced joint malformations. Animal models that accurately recapitulate congenital Zika syndrome are urgently needed for vaccine development and for the study of ZIKV pathogenesis. As guinea pigs have successfully been used to model transplacental infections by cytomegalovirus, syphilis, and Listeria monocytogenes, we sought to test whether ZIKV could productively infect guinea pigs and whether viral transmission with attendant fetal pathology would occur after a mid-gestation viral challenge. We found that guinea pig cells supported ZIKV replication in vitro. Experimental infection of non-pregnant animals did not result in overt disease but low-level, detectable viremia was observed. When pregnant guinea pigs were challenged with ZIKV at between 18 and 21 days gestational age, ZIKV was not detected in maternal or pup blood, plasma, or tissues and no significant differences in m...Continue Reading

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Citations

Oct 17, 2018·The Journal of General Virology·Lindomar José PenaAntonio Carlos de Freitas
Mar 15, 2018·Epidemiology and Infection·C Talero-GutiérrezA de-la-Torre
Jul 24, 2020·Human Vaccines & Immunotherapeutics·Stephen J Thomas, Alan Barrett
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Apr 18, 2019·Expert Opinion on Drug Discovery·Cristine Marie Yde OhkiPatricia Cristina Baleeiro Beltrão-Braga
Oct 23, 2020·PLoS Neglected Tropical Diseases·Harish NarasimhanSabra L Klein
Jul 3, 2021·Frontiers in Immunology·Zachary W BerkebileCraig J Bierle
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Sep 1, 2021·Expert Opinion on Drug Discovery·Cecilia BenazzatoPatricia Cristina Baleeiro Beltrão-Braga

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Methods Mentioned

BETA
ELISA
RNA Assay

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