Assessment and significance of protein-protein interactions during development of protein biopharmaceuticals

Biophysics Reviews
Sandeep YadavSteven J Shire

Abstract

Early development of protein biotherapeutics using recombinant DNA technology involved progress in the areas of cloning, screening, expression and recovery/purification. As the biotechnology industry matured, resulting in marketed products, a greater emphasis was placed on development of formulations and delivery systems requiring a better understanding of the chemical and physical properties of newly developed protein drugs. Biophysical techniques such as analytical ultracentrifugation, dynamic and static light scattering, and circular dichroism were used to study protein-protein interactions during various stages of development of protein therapeutics. These studies included investigation of protein self-association in many of the early development projects including analysis of highly glycosylated proteins expressed in mammalian CHO cell cultures. Assessment of protein-protein interactions during development of an IgG1 monoclonal antibody that binds to IgE were important in understanding the pharmacokinetics and dosing for this important biotherapeutic used to treat severe allergic IgE-mediated asthma. These studies were extended to the investigation of monoclonal antibody-antigen interactions in human serum using the fluore...Continue Reading

References

Jun 20, 1977·Biochemical and Biophysical Research Communications·P D Ross, A P Minton
Jan 26, 1978·Biochimica Et Biophysica Acta·K MuraiT Omae
Dec 1, 1977·Journal of Biochemistry·A FunakoshiY Takagi
May 25, 1977·Journal of Molecular Biology·P D Ross, A P Minton
Nov 1, 1990·Biomedical & Environmental Mass Spectrometry·J T StultsR L Vandlen
Feb 1, 1989·Analytical Biochemistry·A P Minton
Nov 14, 1997·Pharmaceutical Research·J LiuS J Shire
Sep 26, 2001·Scientific American·C Ezzell
Feb 28, 2002·Biophysical Journal·Peter M TessierStanley I Sandler
Sep 28, 2002·Acta Crystallographica. Section D, Biological Crystallography·Kristen DemoruelleW William Wilson
Oct 10, 2002·Journal of the American Chemical Society·Barbara HribarKen A Dill
Jan 25, 1958·Journal of the American Medical Association·G G CASTEN, R J BOUCEK
Sep 26, 2003·Biophysical Journal·J BloustineS Fraden
Mar 27, 2004·Biophysical Chemistry·Ian K MacGregorThomas M Laue
May 5, 2004·Journal of Pharmaceutical Sciences·Steven J ShireJun Liu
Jul 1, 1994·Acta Crystallographica. Section D, Biological Crystallography·A George, W W Wilson
Nov 27, 2004·Nature·Anna StradnerPeter Schurtenberger
Sep 26, 2006·Journal of Pharmaceutical Sciences·Wei WangSandeep Nema
Jan 30, 2007·Biophysical Chemistry·Tsutomu ArakawaSerge N Timasheff
Mar 26, 2008·Proceedings of the National Academy of Sciences of the United States of America·Anuj ShuklaDmitri I Svergun
Jun 25, 2008·Annual Review of Biophysics·Huan-Xiang ZhouAllen P Minton
Aug 26, 2009·Journal of Pharmaceutical Sciences·Sandeep YadavDevendra S Kalonia
Aug 27, 2010·Journal of Pharmaceutical Sciences·Satish Kumar Singh
Sep 8, 2010·Journal of Pharmaceutical Sciences·Sandeep YadavDevendra S Kalonia
Sep 21, 2010·The Journal of Physical Chemistry. B·Thomas M SchererAllen P Minton

❮ Previous
Next ❯

Citations

Sep 17, 2020·MAbs·Yvonne ChenNicholas J Agard
Nov 5, 2019·Journal of Pharmaceutical Sciences·Thomas M Laue, Steven J Shire
Oct 29, 2019·The Journal of Physical Chemistry. a·Aleksandra M KusovaYuriy F Zuev

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bioinformatics in Biomedicine

Bioinformatics in biomedicine incorporates computer science, biology, chemistry, medicine, mathematics and statistics. Discover the latest research on bioinformatics in biomedicine here.