Assessment by flow cytometry of intracellular cytokine production in the peripheral blood cells of renal transplant recipients

Clinical Transplantation
Colm C MageeM H Sayegh

Abstract

There is accumulating evidence that non-invasive immune monitoring may be useful in the early period after renal transplant, particularly with regard to predicting the presence of acute rejection. It is less clear whether chronic allograft nephropathy (CAN) is also associated with consistent changes in peripheral blood or urine cells. We hypothesized that patients with CAN would manifest different patterns of cytokine production (compared with non-CAN controls), detectable in peripheral blood mononuclear cells (PBMCs). Flow cytometry was used to quantify production within PBMCs of multiple cytokines. A pilot study showed significant differences in cytokine production between healthy controls and transplanted subjects. However, differences between transplanted patients with and without CAN were small and non-significant. Flow cytometry is a potentially useful method for quantifying cytokine production by PBMCs of renal transplant recipients. The technique is sensitive enough to detect differences between distinct test groups but could not find differences between recipients with and without CAN. This probably reflects the lack of a true difference because pathological changes within the long-term allograft may simply not be refl...Continue Reading

References

Oct 30, 1998·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·U KunzendorfS Bulfone-Paus
Feb 13, 1999·Kidney International·L C RacusenY Yamaguchi
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Oct 25, 2002·Journal of the American Society of Nephrology : JASN·Behzad NajafianMichael Mauer

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Citations

Nov 20, 2008·Pediatric Transplantation·Udeme D EkongMaurice R G O'Gorman
Dec 11, 2013·Proteomics. Clinical Applications·Diane M CibrikKent J Johnson
Feb 20, 2007·Transplant Immunology·Grégoire WieërsRaymond Reding
Feb 6, 2018·Statistics in Biosciences·Ling ZhouPeter X-K Song
Feb 25, 2006·Liver Transplantation : Official Publication of the American Association for the Study of Liver Diseases and the International Liver Transplantation Society·Raymond RedingDominique Latinne
Apr 8, 2010·Expert Review of Molecular Diagnostics·Valeria R MasDaniel G Maluf

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