Assessment of a rtPCR for the detection of virulent and benign Dichelobacter nodosus, the causative agent of ovine footrot, in Australia

BMC Veterinary Research
Nickala BestTravis Beddoe

Abstract

Ovine footrot is a highly contagious bacterial disease of sheep, costing the Australian sheep industry millions of dollars annually. Dichelobacter nodosus, the causative agent of footrot, is a gram-negative anaerobe classed into virulent and benign strains as determined by thermostability of their respective protesases. Current methods for detection of D. nodosus are difficult and time-consuming, however new molecular techniques capable of rapidly detecting and typing D. nodosus have been reported. A competitive real-time PCR (rtPCR) method, based on the ability to detect a 2 nucleotide difference in the aprV2 (virulent) and aprB2 (benign) extracellular protease gene has been tested on Australian samples for determining detection rates, along with clinically relevant cut-off values and performance in comparison to the traditional culturing methods. The rtPCR assay was found to have a specificity of 98.3% for virulent and 98.7% for benign detection from samples collected. Sheep with clinical signs of footrot showed a detection rate for virulent strains of 81.1% and for benign strains of 18.9%. A cut-off value of a Ct of 35 was found to be the most appropriate for use in Victoria for detection of sheep carrying virulent D. nodosu...Continue Reading

References

Apr 1, 1971·Journal of Comparative Pathology·J R Egerton, D S Roberts
May 1, 1968·Australian Veterinary Journal·N P Graham, J R Egerton
Apr 1, 1969·Journal of Comparative Pathology·J R EgertonI M Parsonson
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Nov 7, 2017·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Deborah GreberAdrian Steiner

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Citations

May 1, 2021·Animals : an Open Access Journal From MDPI·Julia StormsOttmar Distl

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Methods Mentioned

BETA
PCR

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Excel
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