Assessment of AMPK-Stimulated Cellular Long-Chain Fatty Acid and Glucose Uptake

Methods in Molecular Biology
Joost J F P LuikenMiranda Nabben

Abstract

Here we describe an assay for simultaneous measurement of cellular uptake rates of long-chain fatty acids (LCFA) and glucose that can be applied to cells in suspension. The uptake assay includes the use of radiolabeled substrates at such concentrations and incubation periods that exact information is provided about unidirectional uptakes rates. Cellular uptake of both substrates is under regulation of AMPK. The underlying mechanism includes the translocation of LCFA and glucose transporters from intracellular membrane compartments to the cell surface, leading to an increase in substrate uptake. In this chapter, we explain the principles of the uptake assay before detailing the exact procedure. We also provide information of the specific LCFA and glucose transporters subject to AMPK-mediated subcellular translocation. Finally, we discuss the application of AMPK inhibitors and activators in combination with cellular substrate uptake assays.

Related Concepts

Related Feeds

Cardiovascular Homeostasis

Cardiovascular regulation and homeostasis is important in maintaining health and dysfunction may lead to cardiovascular diseases. Nitric oxide and vascular endothelium are one of many molecules involved in the homeostatic mechanism. Here is the latest research on cardiovascular homeostasis.

Related Papers

Biochemical and Biophysical Research Communications
Daphna D J HabetsJoost J F P Luiken
Canadian Journal of Applied Physiology = Revue Canadienne De Physiologie Appliquée
Joost J F P LuikenArend Bonen
© 2022 Meta ULC. All rights reserved