Assessment of arsenic-induced modifications in the DNA methylation of insulin-related genes in rat pancreatic islets

Ecotoxicology and Environmental Safety
Fazlullah KhanMohammad Abdollahi

Abstract

Extended exposure to inorganic arsenic through contaminated drinking water has been linked with increased incidence of diabetes mellitus. The most common exposure occurs through the consumption of contaminated drinking water mainly through geogenic sources of inorganic arsenic. Epigenetic modifications are important mechanisms through which environmental pollutants could exert their toxic effects. Bisulfite sequencing polymerase chain reaction method followed by Sanger sequencing was performed for DNA methylation analysis. Our results showed that sodium arsenite treatment significantly reduced insulin secretion in pancreatic islets. It was revealed that the methylation of glucose transporter 2 (Glut2) gene was changed at two cytosine-phosphate-guanine (CpG) sites (-1743, -1734) in the promoter region of the sodium arsenite-treated group comparing to the control. No changes were observed in the methylation status of peroxisome proliferator-activated receptor-gamma (PPARγ), pancreatic and duodenal homeobox 1 (Pdx1) and insulin 2 (Ins2) CpG sites in the targeted regions. Measuring the gene expression level showed increase in Glut2 expression, while the expression of insulin (INS) and Pdx1 were significantly affected by sodium arse...Continue Reading

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Citations

Dec 30, 2020·Biomolecules·Clémence RivesWalter Wahli
Feb 11, 2021·Diabetes, Metabolic Syndrome and Obesity : Targets and Therapy·Jiangman LiuJingshan Shi
May 10, 2021·Molecular Aspects of Medicine·David J Hill

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