PMID: 8938486Nov 1, 1996Paper

Assessment of aspects of the toxicity of Clostridium perfringens epsilon-toxin using the MDCK cell line

Human & Experimental Toxicology
C D Lindsay

Abstract

1. The epithelial Madin Darby Canine Kidney (MDCK) cell line was used to study the toxicity of epsilon-toxin from Clostridium perfringens. The epithelial MDCK cell line is known to be sensitive to epsilon-toxin of Clostridium perfrigens and to investigate its mechanism of action, the neutral red assay has been used to dermine the viability of cultures of this cell line. 2. Comparison of the LC50s obtained at 34 degrees C and 0 degree C showed that the lethality of epsilon-toxin was reduced by 18-fold at the lower temperature. The effect of temperature on epsilon-toxin lethality is unlikely to be due to reductions in membrane fluidity for the addition of Ca2+ or Mg2+ (2 mM) to buffer containing toxin was without effect. Varying the pH of the toxin-containing buffer from 6.9 to 8.7 did not increase the lethality of the toxin, though the most acidic pH used (5.8) was found to potentiate its action on MDCK cells. 3. The effect of inhibiting endocytosis on the lethality of epsilon-toxin was also investigated by incubating cultures of MDCK cells with and without sodium azide over a range of concentrations of toxin. The co-administration of sodium azide did not reduce the toxicity of epsilon-toxin, suggesting that energy-dependent upt...Continue Reading

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Citations

Feb 13, 2003·Comparative Immunology, Microbiology and Infectious Diseases·Gianfranco DonelliRichard W Titball
Sep 11, 2001·FEMS Immunology and Medical Microbiology·E BorrmannH Köhler
Oct 13, 2012·Chemical Reviews·Ekaterina M Nestorovich, Sergey M Bezrukov
May 22, 2010·Human & Experimental Toxicology·Mariano E Fernandez MiyakawaClaudia Silberstein
Jun 23, 2004·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Alex Soler-JoverMireia Martín-Satué
Dec 23, 2003·Human & Experimental Toxicology·Dominic R BealChristopher D Lindsay
Jun 10, 2003·Journal of Applied Toxicology : JAT·Brian M Paddle
Apr 27, 2011·The FEBS Journal·Monika Bokori-BrownRichard W Titball
Sep 8, 2019·Disease Models & Mechanisms·Abhishek ShivappagowdarShailja Singh
Jun 16, 2019·Nature Communications·Christos G SavvaMonika Bokori-Brown
Jun 7, 2005·Advanced Drug Delivery Reviews·Nicholas J Mantis

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