Assessment of bystander killing-mediated therapy of malignant brain tumors using a multimodal imaging approach

Stem Cell Research & Therapy
Cindy LetenUwe Himmelreich

Abstract

In this study, we planned to assess if adult stem cell-based suicide gene therapy can efficiently eliminate glioblastoma cells in vivo. We investigated the therapeutic potential of mouse Oct4(-) bone marrow multipotent adult progenitor cells (mOct4(-) BM-MAPCs) in a mouse glioblastoma model, guided by multimodal in vivo imaging methods to identify therapeutic windows. Magnetic resonance imaging (MRI) of animals, wherein 5 × 10(5) syngeneic enhanced green fluorescent protein-firefly luciferase-herpes simplex virus thymidine kinase (eGFP-fLuc-HSV-TK) expressing and superparamagnetic iron oxide nanoparticle labeled (1 % or 10 %) mOct4(-) BM-MAPCs were grafted in glioblastoma (GL261)-bearing animals, showed that labeled mOct4(-) BM-MAPCs were located in and in close proximity to the tumor. Subsequently, ganciclovir (GCV) treatment was commenced and the fate of both the MAPCs and the tumor were followed by multimodal imaging (MRI and bioluminescence imaging). In the majority of GCV-treated, but not phosphate-buffered saline-treated animals, a significant difference was found in mOct4(-) BM-MAPC viability and tumor size at the end of treatment. Noteworthy, in some phosphate-buffered saline-treated animals (33 %), a significant decrea...Continue Reading

References

Mar 5, 1996·Proceedings of the National Academy of Sciences of the United States of America·M MesnilH Yamasaki
Nov 6, 2004·Journal of Neuro-oncology·E Antonio ChioccaMartine L M Lamfers
Mar 11, 2005·The New England Journal of Medicine·Roger StuppUNKNOWN National Cancer Institute of Canada Clinical Trials Group
Jul 6, 2006·Molecular Therapy : the Journal of the American Society of Gene Therapy·Christophe M DerooseVeerle Baekelandt
Oct 19, 2006·Experimental Hematology·April BreyerYuehua Jiang
Apr 26, 2007·Molecular Therapy : the Journal of the American Society of Gene Therapy·Hrvoje MileticDorothee von Laer
Jun 15, 2007·PloS One·Alexandra WinkelerAndreas H Jacobs
Jul 10, 2007·Cancer Research·Lucia KucerovaCestmir Altaner
Jul 10, 2007·Acta Neuropathologica·David N LouisPaul Kleihues
Nov 17, 2007·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Hrvoje MileticDorothee von Laer
May 10, 2008·Minimally Invasive Therapy & Allied Technologies : MITAT : Official Journal of the Society for Minimally Invasive Therapy·Uwe Himmelreich, Mathias Hoehn
Sep 22, 2009·Cancer Letters·Miroslava MatuskovaLucia Kucerova
Oct 27, 2009·Experimental Hematology·Ariane LuyckxAn D Billiau
Aug 16, 2011·Journal of Nuclear Medicine : Official Publication, Society of Nuclear Medicine·Wolf-Dieter HeissHeinrich Lanfermann
Dec 15, 2011·Neuro-oncology·Emanuela Binello, Isabelle M Germano
May 29, 2012·Cancer Letters·Sónia DuarteValérie Pierrefite-Carle
Jun 13, 2012·Journal of Nuclear Medicine : Official Publication, Society of Nuclear Medicine·Thomas VielAndreas H Jacobs
Feb 5, 2013·Nature·Heidi BraumüllerMartin Röcken
May 7, 2013·Applied Microbiology and Biotechnology·Nuno BernardesArsenio M Fialho
May 23, 2013·Pharmacology & Therapeutics·Tareq A JuratliDietmar Krex
Aug 24, 2013·Critical Reviews in Microbiology·Chao Zu, Jiansheng Wang

❮ Previous
Next ❯

Citations

Feb 14, 2021·Materials Science & Engineering. C, Materials for Biological Applications·Barbara Blanco-FernandezElisabeth Engel

❮ Previous
Next ❯

Methods Mentioned

BETA
xenograft
imaging techniques
bioluminescence imaging
imaging methods

Software Mentioned

Image J
Paravision
GraphPad
living
GraphPad Prism

Related Concepts

Related Feeds

Basal Ganglia

Basal Ganglia are a group of subcortical nuclei in the brain associated with control of voluntary motor movements, procedural and habit learning, emotion, and cognition. Here is the latest research.

CREs: Gene & Cell Therapy

Gene and cell therapy advances have shown promising outcomes for several diseases. The role of cis-regulatory elements (CREs) is crucial in the design of gene therapy vectors. Here is the latest research on CREs in gene and cell therapy.

Cancer Imaging

Imaging techniques, including CT and MR, have become essential to tumor detection, diagnosis, and monitoring. Here is the latest research on cancer imaging.

Cancer Stem Cells in Glioblastoma

Glioblastoma is the most common and aggressive type of brain tumor. It contains a population of tumor initiating stem cell-like cells known as cancer stem cells. Investigations are ongoing into these cancer stem cells found in these solid tumors which are highly resistance to treatment. Here is the latest research on cancer stem cells in glioblastoma.