Assessment of Chitosan-Based Hydrogel and Photodynamic Inactivation against Propionibacterium acnes

Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry
Maria Lucia FradeCarla Raquel Fontana

Abstract

Chitosan (CH) is a biopolymer that exhibits a number of interesting properties such as anti-inflammatory and antibacterial activity and is also a promising platform for the incorporation of photosensitizing agents. This study aimed to evaluate the efficacy of antimicrobial activity of chitosan hydrogel formulation alone and in combination with the methylene blue (MB) associated with antimicrobial photodynamic therapy (aPDT) against planktonic and biofilm phase of Propionibacterium acnes. Suspensions were sensitized with 12.5, 25.0, 37.5, 50.0 μg/mL of MB for 10 min and biofilms to 75, 100 and 150 μg/mL for 30 min then exposed to red light (660 nm) at 90 J/cm² and 150 J/cm² respectively. After treatments, survival fractions were calculated by counting the number of colony-forming units. The lethal effect of aPDT associated with CH hydrogel in planktonic phase was achieved with 12.5 µg/mL MB and 1.9 log10 biofilm reduction using 75 µg/mL MB. Rheological studies showed that formulations exhibited pseudoplastic non-Newtonian behavior without thixotropy. Bioadhesion test evidenced that the formulations are highly adhesive to skin and the incorporation of MB did not influence the bioadhesive force of the formulations.

References

Jan 5, 2002·Advanced Drug Delivery Reviews·Allan S Hoffman
Sep 19, 2003·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·John D SmartWilliam R Wilber
Jul 30, 2004·International Journal of Food Microbiology·Hui LiuLiping Sun
Feb 18, 2005·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·C OpricaUNKNOWN ESCMID Study Group on Antimicrobial Resistance in Anaerobic Bacteria
Nov 4, 2006·Advanced Drug Delivery Reviews·Chien-Chi Lin, Andrew T Metters
Dec 26, 2006·Journal of Biomedical Materials Research. Part a·Roger BaystonBryar Nuradeen
May 6, 2008·Applied and Environmental Microbiology·Dina RaafatHans-Georg Sahl
Aug 21, 2008·Drug Delivery·Carla Raquel FontanaRosemary Adriana Chiérici Marcantonio
Apr 28, 2009·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·A HolmbergM Rasmussen
Jul 9, 2009·Biotechnology Advances·Mi Lan KangHan Sang Yoo
Jul 16, 2009·Journal of Periodontal Research·C R FontanaN S Soukos
Jan 20, 2010·Clinics in Dermatology·Clio Dessinioti, Andreas D Katsambas
Mar 2, 2010·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·Taís GratieriRenata Fonseca Vianna Lopez
Sep 24, 2010·Photochemistry and Photobiology·Anil KishenMichael R Hamblin
Oct 19, 2010·International Journal of Food Microbiology·Ming KongHyun Jin Park
Mar 1, 2009·Microbial Biotechnology·Dina Raafat, Hans-Georg Sahl
Jan 1, 2010·International Journal of Molecular Sciences·Bae Keun Park, Moon-Moo Kim
Jun 7, 2011·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·Taís GratieriRenata F V Lopez
Sep 2, 2011·Lancet·Hywel C WilliamsSarah Garner
Dec 21, 2011·Drug Development and Industrial Pharmacy·Dong-Min ParkChong-Kook Kim
Feb 24, 2012·The British Journal of Dermatology·A C JahnsO A Alexeyev
Apr 21, 2012·Journal of Biomedical Nanotechnology·Flávia Chiva CarvalhoMaria Palmira Daflon Gremião
Aug 3, 2013·Journal of the European Academy of Dermatology and Venereology : JEADV·C BeylotB Dreno
Jun 6, 2014·Clinical, Cosmetic and Investigational Dermatology·Marilyn T Wan, Jennifer Y Lin
Jul 2, 2014·Experimental Dermatology·Anika C Jahns, Oleg A Alexeyev
Sep 2, 2014·Photodiagnosis and Photodynamic Therapy·Sung Sook ChoiHiun Suk Chae
May 16, 2015·Pharmaceutical Development and Technology·Giovana CalixtoMarlus Chorilli
Aug 11, 2015·International Journal of Nanomedicine·Rafael SalmaziMarlus Chorilli
Jan 29, 2016·Biomaterials Science·Santiago GrijalvoDavid Díaz Díaz
Feb 24, 2016·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Giovana Aparecida de Souza CintraMarlus Chorilli
Mar 14, 2016·Journal of Photochemistry and Photobiology. B, Biology·Maria Rita RonquiCarla Raquel Fontana
Aug 20, 2016·Dermatology and Therapy·Theresa N CanavanBoni E Elewski
Oct 13, 2016·Expert Opinion on Drug Delivery·Lisa ElviriRuggero Bettini
Jun 18, 2017·Photomedicine and Laser Surgery·Tatiana Maria Starck Fogaça de Aguiar ColettiCarla Raquel Fontana
Nov 5, 2017·Photodiagnosis and Photodynamic Therapy·Mariana Alencar NemezioSilmara Aparecida Milori Corona

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Citations

Jul 25, 2019·Macromolecular Bioscience·Alexander DartPeter Kingshott
Nov 16, 2019·Nanoscale·Ruirui XingXuehai Yan
Jul 17, 2019·AAPS PharmSciTech·Giovana Maria Fioramonti CalixtoCarla Raquel Fontana
Aug 29, 2019·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Bhavya KhuranaMathias O Senge
Dec 12, 2018·Advanced Materials·Xingshu LiXi Zhang
Oct 25, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Eniko ManekGeorg A Petroianu
Feb 11, 2021·Materials·Lucie Pierau, Davy-Louis Versace
Nov 15, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·András KelemenKatalin Kristó
Mar 27, 2021·Carbohydrate Polymers·Victor Hugo Sousa AraujoMarlus Chorilli
Apr 26, 2021·International Journal of Pharmaceutics·Gabriela Corrêa CarvalhoMarlus Chorilli
Jul 2, 2021·Photodiagnosis and Photodynamic Therapy·Carolina SanteziLívia Nordi Dovigo
Jan 14, 2021·Journal of Agricultural and Food Chemistry·Luciano Dibona-Villanueva, Denis Fuentealba
Nov 16, 2020·International Journal of Pharmaceutics·Amanda Letícia Polli SilvestreMarlus Chorilli

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Methods Mentioned

BETA
rheology

Software Mentioned

GraphPad
Graph Pad Prism

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