PMID: 45493Sep 1, 1976

Assessment of clinical laboratory techniques in the measurement of oxygen saturation in whole blood

R SarmaJ L Johnson


The accuracy and advantages of determining percentage of hemoglobin oxygen saturation of whole blood by advanced instruments of oximetry, gas chromatography, and the computation of oxygen saturation from pO2 and pH data are compared with the Van Slyke-Neill manometric method. It was found that oximetry is extremely fast, less expensive, and more accurate than in the past, the apparatus requires little training to operate and has diminutive service maintenance. The gas chromatograph proved to be highly accurate and could serve as a standard calibration check method in place of the more time-consuming Van Slyke-Neill apparatus for oximetry and pO2 and pH electrode instrumentation. Computation of O2 saturation from pO2 and pH data also proved to be accurate, confirming that this method is more useful during cardiac surgery because it can provide additional acid-base information. In the low oxygen saturation range (less than 60%), all three methods proved to be of similar sensitivity.


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May 1, 1966·Journal of Applied Physiology·J W SEVERINGHAUS
Nov 1, 1958·Journal of Applied Physiology·J W SEVERINGHAUS, A F BRADLEY
Jan 1, 1961·The American Journal of Physiology·D M GOMEZ
Mar 1, 1961·Journal of Applied Physiology·D S LUKAS, S M AYRES
Mar 1, 1961·Journal of Applied Physiology·R H WILSONE HIGGINS
Sep 1, 1964·Journal of Applied Physiology·J HEDLEY-WHYTE, M B LAVER
Jan 1, 1965·Journal of Applied Physiology·J F NUNNA J COLEMAN
Jun 1, 1965·Analytical Chemistry·H Y YEE
Mar 1, 1960·Journal of Applied Physiology·B E JAY, R H WILSON

Related Concepts

Whole Blood
Molecular Oxygen Saturation
Discipline of Heart Surgery
Oxygen Saturation Measurement
Whole Blood Specimen
Chromatography, Gas-Liquid
Specimen Type - Electrode
Arterial Blood Gases % Oxygen Saturation (Lab Test)

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