Assessment of cochlear function in mice: distortion-product otoacoustic emissions.

Current Protocols in Neuroscience
G K MartinB L Lonsbury-Martin

Abstract

Distortion-product otoacoustic emissions (DPOAEs) can be measured in the ear canal following the presentation of two tones. These emissions are generated by the outer hair cells (OHCs) of the inner ear and they are reduced or absent when the OHCs are damaged by, for example, exposure to excessive noise or ototoxic drugs. Consequently, DPOAEs provide a powerful and noninvasive means to assess the robustness of OHC function. A detailed method is described for measuring DPOAEs to assess cochlear function in mice. Recommendations are given for the required equipment and instructions are presented for setting up a DPOAE system. Also, a protocol is outlined for measuring DPOAEs in mice and troubleshooting tips are provided. Examples of data analysis procedures following noise exposure in mice are included, as well. These methods are not only applicable to mice, but can be performed using essentially all small laboratory animals.

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Citations

Feb 5, 2010·Journal of Toxicology·B PouyatosL D Fechter
Mar 13, 2015·Frontiers in Aging Neuroscience·Lorena SanzCarlos Avendaño
Oct 14, 2017·Journal of the Association for Research in Otolaryngology : JARO·Abhilash PonnathJennifer J Lentz
Jan 1, 2011·Current Protocols in Mouse Biology·Neil J InghamKaren P Steel
Jan 16, 2007·Hearing Research·Benoît PouyatosLaurence Fechter
Apr 4, 2008·Hearing Research·Devrim BektasBrenda L Lonsbury-Martin

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