Assessment of contact allergens by dissociation of irritant and sensitizing properties

Toxicology in Vitro : an International Journal Published in Association with BIBRA
John J L JacobsGraham R Elliott

Abstract

The human organotypic skin explant culture (hOSEC) model is a promising alternative in vitro model for screening contact allergens. In this model, the chemical-induced migration of Langerhans cells (LCs) out of the epidermis, evaluated after a 24-h exposure period, is used as a measure of sensitizer potential. As skin irritants can also induce LC migration it is essential that concentrations of test chemicals are used that are not even weakly irritant. Using the hOSEC irritation model chemicals are classified as weak irritants if they are toxic after a 48-h exposure period. Toxicity is determined by methyl green-pyronine (MGP) staining of hOSEC. We studied three frequently used non-sensitizing skin irritants and six potent or frequent human sensitizers in a dose-response. A complete discrimination between non-sensitizers and contact sensitizers was obtained for the chemicals tested when the concentrations used were lower than the weak irritant concentrations. Frequency of positive allergen reactions in patch test of human populations correlated with the difference between weak irritant concentrations and the lowest concentration inducing significant LC migration. Sensitizer potency correlated with chemical irritancy as determin...Continue Reading

References

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Jan 11, 2001·Journal of Immunological Methods·J J JacobsG R Elliott
Sep 11, 2002·Toxicology in Vitro : an International Journal Published in Association with BIBRA·J J L JacobsG R Elliott

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Citations

Jun 1, 2012·Expert Review of Dermatology·James Varani
Nov 12, 2010·The Journal of Investigative Dermatology·Krista OuwehandSusan Gibbs
Feb 7, 2013·The British Journal of Dermatology·J P McFaddenI Kimber
Jul 13, 2006·Alternatives to Laboratory Animals : ATLA·Monika Schäfer-KortingAlexander Vuia
Mar 7, 2007·The Journal of Immunology : Official Journal of the American Association of Immunologists·Sofia I BogiatziVassili Soumelis

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