Assessment of cytology based molecular analysis to guide targeted therapy in advanced non-small-cell lung cancer

Oncotarget
Wenbin LiJianming Ying

Abstract

To investigate the use of molecular testing on cytological specimens in selecting advanced non-small cell lung cancer (NSCLC) patients who are adequate for targeted treatment, a total of 137 NSCLC cases were analyzed by fluorescence in situ hybridization (FISH) for anaplastic lymphoma kinase (ALK) rearrangements, and Epidermal growth factor receptor (EGFR), kirsten rat sarcoma viral oncogene homolog (KRAS) mutations were evaluated by quantitative real-time PCR (qRT-PCR) platform combining amplification refractory mutation system (ARMS) primers and TaqMan probes. Cytological specimens included 91 fine-needle aspirates, 5 fibreoptic bronchoscopic derived samples and 41 pleural effusions. Among 137 NSCLCs analyzed for ALK FISH, 16 (11.7%, of 137) were detected to harbor ALK rearrangement. FISH positive cases were all defined as adenocarcinoma (ADC) histologic subtype and the FNA samples showed the highest ALK positive rate (13.2%, 12/91). Of the 9 ALK FISH positive patients who received crizotinib treatment, 8 (88.9%) patients exhibited tumor regression. In addition, 60 (44.8%, of 134) cases were found to harbor EGFR mutations and 22 patients with EGFR sensitive mutations who received gefitinib or erlotinib treatment showed a medi...Continue Reading

References

Apr 23, 2009·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Kengo TakeuchiHiroyuki Mano
Aug 21, 2009·The New England Journal of Medicine·Rafael RosellUNKNOWN Spanish Lung Cancer Group
Sep 9, 2011·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Christopher G AzzoliUNKNOWN American Society of Clinical Oncology
Sep 29, 2011·Journal of Clinical Pathology·Umberto MalapelleGiancarlo Troncone
Jul 19, 2012·Cytopathology : Official Journal of the British Society for Clinical Cytology·B PangM Salto-Tellez
Oct 3, 2012·Lung Cancer : Journal of the International Association for the Study of Lung Cancer·Fumihiro YamaguchiKunihiko Fukuchi
Dec 5, 2012·Acta Cytologica·Edmond S K MaChris L P Wong
Apr 5, 2013·Cytopathology : Official Journal of the British Society for Clinical Cytology·M J NeatG Santis
Jun 4, 2013·The New England Journal of Medicine·Alice T ShawPasi A Jänne
Aug 2, 2013·Annals of Oncology : Official Journal of the European Society for Medical Oncology·J YingN Lu
Apr 4, 2014·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Miguel A Molina-VilaRafael Rosell
Mar 11, 2015·Cytopathology : Official Journal of the British Society for Clinical Cytology·S BravacciniM Puccetti
May 23, 2015·Journal of Thoracic Oncology : Official Publication of the International Association for the Study of Lung Cancer·Ling ShanJianming Ying
Jul 5, 2015·Lung Cancer : Journal of the International Association for the Study of Lung Cancer·Ling ShanJianming Ying

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Citations

Jul 26, 2017·Cancer Cytopathology·Pasquale PisapiaGiancarlo Troncone
Mar 27, 2018·Diagnostic Cytopathology·Oscar LinS Joseph Sirintrapun
Aug 2, 2021·Cytopathology : Official Journal of the British Society for Clinical Cytology·Fang ZhouAndre L Moreira

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Methods Mentioned

BETA
biopsy
PCR
DNA assay

Software Mentioned

SPSS
GATK

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