Assessment of cytotoxicity and genotoxicity of stem bark extracts from Canarium odontophyllum Miq. (dabai) against HCT 116 human colorectal cancer cell line

BMC Complementary and Alternative Medicine
Dayang Fredalina BasriKok Meng Chan

Abstract

Canarium odontophyllum Miq. is a plant species widely known as 'dabai' and can be vastly found in Sarawak. The aim of this study was to assess the cytotoxic and genotoxic effect of extracts from stem bark of C. odontophyllum against HCT 116 human colorectal cancer cell line. The IC50 values of the aqueous, methanol, and acetone extracts against HCT 116 cells as well as the acetone extract against human colon fibroblast cell line CCD-18co were determined using the MTT assay. The concentration of the extracts ranged from 12.5 to 200 μg/ml at treatment time of 24, 48 and 72 h. Annexin V-FITC/PI labelling assay was employed to determine mode of HCT 116 cell death induced by acetone extract at 48 h. The DNA damage induced by the extract in HCT 116 cells was detected using alkaline comet assay at 30 min of IC10 and IC25 treatment. Acetone extract exhibited the highest cytotoxic effect against HCT 116 cells compared to methanol and aqueous extract at 24, 48 and 72 h. Despite no cytotoxic effect by acetone extract against CCD-18co cells at 24 and 48 h, however at 72 h, CCD-18co cells proliferated. Apoptosis assessment using Annexin V-FITC/PI labelling assay revealed that the primary cell death was via apoptosis after 48 h treatment. Lo...Continue Reading

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Citations

Mar 29, 2018·Archives of Physiology and Biochemistry·Hadi BamehrRezvan Najafi
May 12, 2018·Future Medicinal Chemistry·Bruna C ZorzanelliBruno K Robbs
Jan 31, 2018·BMC Complementary and Alternative Medicine·Victor KueteHülya Sivas
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Feb 29, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Suchanuch OndeePiyanuch Rojsanga

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Datasets Mentioned

BETA
UKMB
40052

Methods Mentioned

BETA
ELISA
flow cytometry
electrophoresis

Software Mentioned

Cell Quest

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Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

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