Assessment of differential cytokine effects on angiogenesis using an in vivo model of cutaneous wound repair

The Journal of Surgical Research
J F Roesel, L B Nanney

Abstract

Angiogenesis, or new blood vessel formation, has been a subject of intense investigation in recent years. A major obstacle in this research has been the selection of an appropriate in vivo model with which comparisons to humans can be made as well as a reliable quantitative method. Using the porcine excisional wound healing model, we report a new and simple technique for obtaining objective assessments of the microvascular compartment. Factor VIII immunostaining of histological specimens was utilized for specific identification of endothelium devoid of background interference. This technique was coupled with morphometric analysis to quantitate the differential effects of tumor necrosis factor alpha (TNF alpha), transforming growth factor beta (TGF beta), basic fibroblast growth factor (bFGF), insulin-like growth factor-1 (IGF-1), and epidermal growth factor (EGF) within healing porcine wounds. All cytokines stimulated angiogenesis, with low dose TNF alpha and bFGF treatments exhibiting the most profound effects at 7 days postwounding. With increasing levels of TNF alpha (1 ng, 10 ng, 100 ng, and 2.5 micrograms), a step-wise decrease in microvascular area was noted. Although no significant dose responsive differences in angiogen...Continue Reading

Citations

May 16, 2000·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·L B NanneyJ M Davidson
May 15, 2001·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·T P SullivanP Mertz
Dec 31, 1997·Journal of Periodontal Research·S TakayamaH Okada
Mar 5, 2011·Arteriosclerosis, Thrombosis, and Vascular Biology·Giovanni LigrestiRoberto F Nicosia
Mar 1, 2005·Journal of Periodontology·Varawan Sae-LimJennifer Neo
Nov 25, 2006·The Journal of Investigative Dermatology·Martina I OkwuezeLillian B Nanney
Mar 13, 2003·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Scott A HeinrichElizabeth J Kovacs
Mar 14, 2007·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Richard BottLillian B Nanney
Aug 22, 2000·The Journal of Investigative Dermatology·R M DevalarajaA Richmond
Apr 27, 2017·Plastic and Reconstructive Surgery·S Morteza Seyed JafariAmiq Gazdhar
Jun 19, 2003·The Journal of Trauma·Nicholas N NissenLuisa A DiPietro
Jan 26, 2002·Journal of Dental Research·S TakayamaH Okada
Feb 22, 2002·International Journal of Cancer. Journal International Du Cancer·Irene PeroulisMary Saleh
Aug 9, 2003·Critical Care Medicine·Rhonda S FishelAdrian Barbul
Nov 8, 2001·Plastic and Reconstructive Surgery·L L BennettL B Nanney
Jun 26, 2004·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Asim KhanCho Y Pang
Sep 24, 2004·International Journal of Cancer. Journal International Du Cancer·Yoshiharu TomitaNaofumi Mukaida
Jun 30, 2009·Tissue Engineering. Part a·Alan J HusseySandra J Feeney
May 25, 2005·Hernia : the Journal of Hernias and Abdominal Wall Surgery·M KorenkovM Nagelschmidt
Mar 17, 2010·Archives of Dermatological Research·Alexandra L Varol, Chris D Anderson
Jul 16, 2009·Scandinavian Journal of Medicine & Science in Sports·D BringP Ackermann
Jul 12, 2005·The Journal of Burn Care & Rehabilitation·David G Greenhalgh
Feb 8, 2006·American Journal of Physiology. Heart and Circulatory Physiology·Ning HuangCho Y Pang
Mar 27, 2009·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Anna L SanderJohannes M Frank
Aug 15, 2012·Aesthetic Plastic Surgery·Fulya FindikciogluKenan Atabay
Jun 28, 2002·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Ryoichi MoriNaofumi Mukaida
Nov 3, 2017·PloS One·Ba Reun KimJae Ho Kim
Dec 13, 2003·Endocrine Reviews·Stephanie R EdmondsonChristopher J Wraight
Jan 10, 2009·Journal of Wound Care·L K W Chan
Mar 3, 2005·Journal of Wound Care·D Thompson
Mar 27, 1998·Breast Cancer Research and Treatment·A A Rasmussen, K J Cullen
Sep 15, 2012·The Journal of Craniofacial Surgery·Fulya FindikciogluKenan Atabay
Feb 18, 2004·The Breast : Official Journal of the European Society of Mastology·H KnüpferR Preiss
Jan 9, 2001·The Journal of Investigative Dermatology. Symposium Proceedings·M G TonnesenR A Clark
May 19, 2004·American Journal of Surgery·Julie E Park, Adrian Barbul
Jul 17, 1998·British Journal of Plastic Surgery·D R TheileW A Morrison

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.