Assessment of DNA damages in lymphocytes of agricultural workers exposed to pesticides by comet assay in a cross-sectional study

Biomarkers : Biochemical Indicators of Exposure, Response, and Susceptibility to Chemicals
Graziana IntranuovoGiovanni M Ferri

Abstract

To assess the predictive power of the comet assay in the context of occupational exposure to pesticides. The recruited subjects completed a structured questionnaire and gave a blood sample. Exposure to pesticides was measured by means of an algorithm based on Dosemeci's work (Agricultural Health Study). Approximately 50 images were analyzed for each sample via fluorescence microscopy. The extent of DNA damage was estimated by tail moment (TM) and is the product of tail DNA (%) and tail Length. Crude significant risks (odds ratios, ORs) for values higher than the 75th percentile of TM were observed among the exposed subjects (score > 1). The frequency of some confounding factors (sex, age and smoking) was significantly higher among the exposed workers. A significant dose-effect relationship was observed between TM and exposure score. Significant high-risk estimates (ORs), adjusted by the studied confounding factors, among exposure to pesticides and TM, % tail DNA and tail length were confirmed using unconditional logistic regression models. The adjusted associations (ORs) between the comet parameters and exposure to pesticides were significant. The sensitivity of the comet test was low (41%), the specificity (89%) and the predic...Continue Reading

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Citations

Dec 6, 2018·Biomarkers : Biochemical Indicators of Exposure, Response, and Susceptibility to Chemicals·Giovanni Maria FerriLuigi Vimercati
Apr 26, 2019·Biomarkers : Biochemical Indicators of Exposure, Response, and Susceptibility to Chemicals·Akin CayirHayal Cobanoglu
Mar 29, 2020·International Journal of Environmental Research and Public Health·Luigi VimercatiGiovanni Maria Ferri
Jul 10, 2020·Biomarkers : Biochemical Indicators of Exposure, Response, and Susceptibility to Chemicals·Karashdeep Kaur, Rupinder Kaur
Dec 14, 2019·Environmental Science and Pollution Research International·Ana Paola Balderrama-CarmonaEdgar Felipe Morán-Palacio
Nov 19, 2019·International Journal of Environmental Health Research·Jones A KapelekaPatrick A Ndakidemi
Aug 9, 2020·Frontiers in Public Health·Luigi De MariaLuigi Vimercati
Jan 22, 2020·Current Environmental Health Reports·Cynthia L CurlLuke Montrose
Apr 16, 2020·International Journal of Environmental Research and Public Health·Luigi VimercatiGabriella Serio
Sep 14, 2020·The Science of the Total Environment·Jheneffer Sonara Aguiar RamosDaniela de Melo E Silva
Apr 11, 2021·European Journal of Nutrition·Narimane DjekkounHafida Khorsi-Cauet
Nov 3, 2021·Reviews on Environmental Health·Ibrahim IssahJulius N Fobil

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