PMID: 11342238May 9, 2001Paper

Assessment of DNA strand breakage by comet assay in diabetic patients and the role of antioxidant supplementation

Mutation Research
S SardaşA E Karakaya

Abstract

Diabetes patients often show increased production of reactive oxidative species (ROS) together with vascular complications. The presence of these ROS may lead to increased DNA damage in peripheral blood lymphocytes that may be revealed by the comet assay. To test whether DNA is damaged in diabetes, peripheral blood samples were taken from 30 control individuals and 63 diabetic patients (15 insulin dependent (IDDM) and 48 non-insulin dependent (NIDDM)) and the alkaline comet assay was used to evaluate background levels of DNA damage. Significant differences were detected between control and diabetic patients in terms of frequencies of damaged cells. The extend of DNA migration was greater in NIDDM patients by comparison with IDDM patients which might indicate that IDDM patients are handling more oxidative damage on a regular basis. Smoker individuals had higher frequencies of cells with migration by comparison with the non-smokers in both groups. Also, clear differences between patients on placebo and on Vitamin E supplementation for 12 weeks were observed on the basis of the extend of DNA migration during single cell gel electrophoresis.

References

Mar 1, 1988·Experimental Cell Research·N P SinghE L Schneider
May 1, 1995·The American Journal of Medicine·A GriesmacherG Schernthaner
Feb 17, 1996·Lancet·P DandonaT Nicotera

❮ Previous
Next ❯

Citations

Mar 17, 2005·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Maria Grazia AndreassiEugenio Picano
Aug 15, 2002·Mutation Research·Yildiz DinçerHasan Ilkova
Apr 23, 2003·Molecular and Cellular Endocrinology·Patricia MerkelRiccardo Perfetti
Sep 24, 2011·Oxidative Medicine and Cellular Longevity·Saleh A Bakheet, Sabry M Attia
Feb 25, 2014·Mutation Research. Genetic Toxicology and Environmental Mutagenesis·Monika WitczakJan Wilczyński
Feb 28, 2016·Mutation Research. Genetic Toxicology and Environmental Mutagenesis·B C Gómez-MedaG M Zúñiga-González
Nov 15, 2011·Diabetes Research and Clinical Practice·Suresh K G ShettigarRatnakar K Kulkarni
Nov 14, 2012·Molecular Nutrition & Food Research·Elisabeth MüllnerKarl-Heinz Wagner
Feb 15, 2005·Mutation Research·Recep DemirbagAbdurrahim Kocyigit
Jun 6, 2009·Chemico-biological Interactions·S M AttiaA A Alhaider
Jun 27, 2006·International Journal of Cardiology·Moussa AlkhalafNabila Abdella
Nov 5, 2013·Mutation Research. Reviews in Mutation Research·Andrew CollinsUNKNOWN ComNet project
Jun 28, 2014·Asian Pacific Journal of Cancer Prevention : APJCP·Sudhaa AnandRadha Saraswathy
Sep 30, 2004·Free Radical Research·Alma L RosalesMichael H L Green
Jun 22, 2017·The Journal of Maternal-fetal & Neonatal Medicine : the Official Journal of the European Association of Perinatal Medicine, the Federation of Asia and Oceania Perinatal Societies, the International Society of Perinatal Obstetricians·K Dulasi DurgaParkash Chand
Aug 31, 2002·Environmental and Molecular Mutagenesis·Samantha ManfrediMaria Grazia Andreassi
Nov 28, 2018·Mutagenesis·Olgica MihaljevicSlavica Markovic
Nov 11, 2005·Mutagenesis·Heike HoffmannGünter Speit
Sep 27, 2018·Indian Journal of Ophthalmology·Aditya KelkarWinfried Amoaku
Oct 24, 2002·Coronary Artery Disease·Nicoletta BottoMaria G Andreassi
Jul 30, 2002·The American Journal of Clinical Nutrition·Peter Møller, Steffen Loft
Feb 12, 2021·Antioxidants·Carmen MannucciSebastiano Gangemi

❮ Previous
Next ❯

Related Concepts

Related Feeds

Autoimmune Diabetes & Tolerance

Patients with type I diabetes lack insulin-producing beta cells due to the loss of immunological tolerance and autoimmune disease. Discover the latest research on targeting tolerance to prevent diabetes.