Assessment of endoplasmic reticulum glutathione redox status is confounded by extensive ex vivo oxidation.

Antioxidants & Redox Signaling
Brian M DixonTory M Hagen

Abstract

Glutathione (GSH) and glutathione disulfide (GSSG) form the principal thiol redox couple in the endoplasmic reticulum (ER); however, few studies have attempted to quantify GSH redox status in this organelle. To address this gap, GSH and GSSG levels and the extent of protein glutathionylation were analyzed in rat liver microsomes. Because of the likelihood of artifactual GSH oxidation during the lengthy microsomal isolation procedure, iodoacetic acid (IAA) was used to preserve the physiological thiol redox state. Non-IAA-treated microsomes exhibited a GSH:GSSG ratio between 0.7:1 to 1.2:1 compared to IAA-treated microsomes that yielded a GSH:GSSG redox ratio between 4.7:1 and 5.5:1. The majority of artifactual oxidation occurred within the first 2 h of isolation. Thus, the ER GSH redox ratio is subject to extensive ex vivo oxidation and when controlled, the microsomal GSH redox state is significantly higher than previously believed. Moreover, in vitro studies showed that PDI reductase activity was markedly increased at this higher thiol redox ratio versus previously reported GSH:GSSG ratios for the ER. Lastly, we show by both HPLC and Western blot analysis that ER proteins are highly resistant to glutathionylation. Together, the...Continue Reading

References

Aug 1, 1970·The Journal of General Physiology·P F Duggan, A Martonosi
Aug 5, 1998·Proceedings of the National Academy of Sciences of the United States of America·T M HagenB N Ames
Feb 11, 1999·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·T M HagenA B Ames
Nov 23, 2000·Accounts of Chemical Research·M NarayanH A Scheraga
Mar 30, 2001·The Journal of Biological Chemistry·J SunG Meissner
Jun 1, 2002·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Anthony R SmithTory M Hagen
Dec 18, 2002·The Journal of Biological Chemistry·Melissa SchwallerHiram F Gilbert
Aug 5, 2003·BioFactors·Miklós CsalaGábor Bánhegyi
Apr 1, 1960·The Journal of Biophysical and Biochemical Cytology·L SHARE, R W HANSROTE
Nov 25, 2003·The Journal of Biological Chemistry·Rosemary BassRobert B Freedman
May 27, 2004·The Journal of Biological Chemistry·Silvia Nerini MolteniRoberto Sitia
Jul 16, 2004·The Journal of Biological Chemistry·Seema Chakravarthi, Neil J Bulleid
Oct 21, 2004·Biochemical Society Transactions·L Ellgaard
Dec 18, 2004·Mutation Research·Martin Schröder, Randal J Kaufman
Jul 19, 2005·Biochemical and Biophysical Research Communications·Gábor NardaiPéter Csermely
Sep 22, 2006·Antioxidants & Redox Signaling·Agnes GörlachThomas Kietzmann
Oct 4, 2006·Physiological Reviews·Stefan J Marciniak, David Ron
Feb 10, 2007·The FEBS Journal·Hiderou Yoshida
May 1, 2007·FEBS Letters·Gábor BánhegyiJózsef Mandl

❮ Previous
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Citations

Jan 6, 2009·Proceedings of the National Academy of Sciences of the United States of America·Rosa E HansenJakob R Winther
Oct 7, 2011·The Journal of Biological Chemistry·Nicolas BrandesUrsula Jakob
Nov 28, 2008·The Journal of Biological Chemistry·Svetlana N RadyukWilliam C Orr
May 30, 2009·Antioxidants & Redox Signaling·Feras Hatahet, Lloyd W Ruddock
Dec 17, 2009·Antioxidants & Redox Signaling·Miklós CsalaGábor Bánhegyi
Oct 7, 2009·Antioxidants & Redox Signaling·Montserrat MaríJosé C Fernández-Checa
Feb 9, 2010·Antioxidants & Redox Signaling·Vamsi K Kodali, Colin Thorpe
May 22, 2010·Antioxidants & Redox Signaling·Timothy J Tavender, Neil J Bulleid
Oct 21, 2011·Antioxidants & Redox Signaling·David PimentelMarkus Michael Bachschmid
Jun 21, 2012·Experimental Biology and Medicine·Vladimir L KolossovH Rex Gaskins
Dec 4, 2012·Nutrients·William M JohnsonJohn J Mieyal
May 27, 2010·Redox Report : Communications in Free Radical Research·Daniela RogoffPerrin C White
Aug 2, 2008·Free Radical Research·Magdalena L Circu, Tak Yee Aw
Nov 27, 2015·Antioxidants & Redox Signaling·Dmitry Bilan, Vsevolod Belousov
May 23, 2012·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Dove J KeithTory M Hagen
Apr 10, 2012·Seminars in Cell & Developmental Biology·Magdalena L Circu, Tak Yee Aw
Nov 22, 2015·Biochimica Et Biophysica Acta·Eleonora MercatelliLucia Banci
Jul 23, 2011·Trends in Biochemical Sciences·Neil J Bulleid, Lars Ellgaard
Oct 22, 2008·Toxicology and Applied Pharmacology·Marilyn C HendersonDavid E Williams
Jul 23, 2008·Journal of Neurochemistry·Gábor BánhegyiMiklós Csala
Sep 8, 2011·Alcoholism, Clinical and Experimental Research·Hui TangLaura E Nagy
Jul 26, 2011·Journal of Molecular and Cellular Cardiology·Bradford G Hill, Aruni Bhatnagar
Feb 14, 2015·Free Radical Biology & Medicine·Éva MargittaiGábor Bánhegyi
Oct 6, 2012·Biochimica Et Biophysica Acta·Marcel Deponte
Nov 20, 2013·Redox Biology·Davide MonteroChristian Appenzeller-Herzog
Jun 27, 2012·Biochimica Et Biophysica Acta·Magdalena L Circu, Tak Yee Aw
Jan 23, 2015·Antioxidants & Redox Signaling·Marie-Laure Kleme, Emile Levy
May 7, 2016·Arthritis Research & Therapy·Dres DamgaardClaus H Nielsen
Jan 5, 2010·Free Radical Biology & Medicine·Magdalena L Circu, Tak Yee Aw
Oct 25, 2016·Methods : a Companion to Methods in Enzymology·Justin D La Favor, Arthur L Burnett
Aug 31, 2018·Journal of Cellular Biochemistry·Young-Sun LeeYong J Lee
Nov 7, 2018·Cold Spring Harbor Perspectives in Biology·Ryo Ushioda, Kazuhiro Nagata
Oct 31, 2018·Scientific Reports·Alessio BocediGiorgio Ricci
Jan 1, 2020·Antioxidants & Redox Signaling·Jason M HansenCraig Harris
Nov 21, 2018·Life Science Alliance·Antti MoilanenLloyd W Ruddock
Apr 11, 2018·Antioxidants & Redox Signaling·Juan I SbodioBindu D Paul

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