Assessment of endothelium and inflammatory response at the onset of reperfusion injury in hand surgery.

Journal of Inflammation
Pranitha KamatEsther Vögelin

Abstract

Activation of the endothelium, complement activation and generation of cytokines are known events during ischemia-reperfusion (I/R) that mediate tissue injury. Our aim was to elucidate their respective participation at the onset of the reperfusion phase. Tourniquet application in hand surgery causes short-term ischemia, followed by reperfusion and was therefore used as the model in this study. Ten patients were included in the study after obtaining informed consent. A tourniquet was placed on the upper arm and inflated to 250 mmHg for 116 ± 16 min, during which the surgery was performed. Venous blood and tissue samples from the surgical area were taken at baseline as well as 0, 2, and 10 min after reperfusion and analyzed for the following parameters: Endothelial integrity and/or activation were analyzed by measuring heparan sulfate and syndecan-1 in serum, and vWF, heparan sulfate proteoglycan as well as CD31on tissue. Complement activation was determined by C3a and C4d levels in plasma, levels of C1-inhibitor in serum, and IgG, IgM, C3b/c, and C4b/c deposition on tissue. Cytokines and growth factors IL-5, IL-6, IL-7, IL-8, IL-10, IL-17, G-CSF, GM-CSF, MCP-1, TNFα, VEGF, and PDGF bb were measured in the serum. Finally, CK-MM l...Continue Reading

References

Sep 1, 1994·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·K IiharaF Hazama
Feb 11, 1998·The Journal of Hand Surgery : Journal of the British Society for Surgery of the Hand·G GermannH U Steinau
Apr 16, 1998·Molecular and Cellular Biochemistry·D C GuteR J Korthuis
Mar 17, 1999·Clinical Orthopaedics and Related Research·L R MohlerD H Gershuni
Aug 29, 2000·Anesthesia and Analgesia·M GirardisA Pasetto
Sep 20, 2000·American Journal of Physiology. Heart and Circulatory Physiology·D H CherwekD A Taylor
Jan 6, 2004·American Journal of Physiology. Heart and Circulatory Physiology·A W Mulivor, H H Lipowsky
Jul 31, 2004·Clinical Science·Ruksana HudaMali Mathru
Jan 5, 2006·The Journal of Experimental Medicine·Ming ZhangMichael C Carroll
Dec 1, 2006·Journal of Leukocyte Biology·Paula K ShiremanLinda M McManus
Apr 10, 2007·Trends in Cardiovascular Medicine·Jurgen W Van TeeffelenHans Vink
Oct 27, 2007·Scandinavian Journal of Clinical and Laboratory Investigation·T Clementsen, O Reikerås
Nov 1, 2007·Nature Reviews. Molecular Cell Biology·Mark R MorganMark D Bass
Oct 7, 2008·Basic Research in Cardiology·Daniel ChappellBernhard F Becker
Feb 16, 2010·Foot and Ankle Surgery : Official Journal of the European Society of Foot and Ankle Surgeons·T O Smith, C B Hing
Jan 25, 2011·Annals of Medicine·Yara Banz, Robert Rieben
Feb 3, 2011·Annals of Medicine·Kati Kinnunen, Seppo Ylä-Herttuala

❮ Previous
Next ❯

Citations

Feb 12, 2013·Redox Report : Communications in Free Radical Research·Ivana BudicTatjana Jevtovic-Stoimenov
Sep 14, 2016·The Journal of Surgical Research·Hassan AlbadawiMichael T Watkins
Jun 5, 2020·Neuropsychiatric Disease and Treatment·Jiaoying JiaYugang Jiang

❮ Previous
Next ❯

Methods Mentioned

BETA
ELISA
coronary artery bypass
biopsies

Software Mentioned

Bio
Imaris
GraphPad
Plex Manager
Prism

Related Concepts

Related Feeds

Arterial-Venous in Development & Disease

Arterial-venous development may play a crucial role in cardiovascular diseases. Here is the latest research.

© 2021 Meta ULC. All rights reserved