Assessment of established techniques to determine developmental and malignant potential of human pluripotent stem cells.

Nature Communications
International Stem Cell Initiative

Abstract

The International Stem Cell Initiative compared several commonly used approaches to assess human pluripotent stem cells (PSC). PluriTest predicts pluripotency through bioinformatic analysis of the transcriptomes of undifferentiated cells, whereas, embryoid body (EB) formation in vitro and teratoma formation in vivo provide direct tests of differentiation. Here we report that EB assays, analyzed after differentiation under neutral conditions and under conditions promoting differentiation to ectoderm, mesoderm, or endoderm lineages, are sufficient to assess the differentiation potential of PSCs. However, teratoma analysis by histologic examination and by TeratoScore, which estimates differential gene expression in each tumor, not only measures differentiation but also allows insight into a PSC's malignant potential. Each of the assays can be used to predict pluripotent differentiation potential but, at this stage of assay development, only the teratoma assay provides an assessment of pluripotency and malignant potential, which are both relevant to the pre-clinical safety assessment of PSCs.

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Citations

May 29, 2020·Stem Cell Research & Therapy·Hua WangLin Liu
Jun 27, 2020·Regenerative Medicine·Francis KaranuLisa Stehno-Bittel
Aug 10, 2019·World Journal of Stem Cells·Li-Ping Liu, Yun-Wen Zheng
Sep 22, 2020·Current Protocols in Stem Cell Biology·Aya ShibamiyaBärbel Maria Ulmer
Sep 16, 2020·Experimental & Molecular Medicine·Yulia PaninaWataru Fujibuchi
Jan 13, 2019·International Journal of Molecular Sciences·João LoboLeendert H J Looijenga
Oct 7, 2020·Nature Communications·Dylan StavishTariq Enver
Dec 29, 2020·Frontiers in Physiology·Ankita Thawani, Andrew K Groves
Oct 22, 2019·Biochimica Et Biophysica Acta. Molecular Cell Research·Ruben A AlexanianTimothy J Kamp

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Datasets Mentioned

BETA
GSE97964

Methods Mentioned

BETA
PCR
xenograft
chromosomal aberrations
RNA-seq
transcription profiling
xenografts
xenografting

Software Mentioned

Cufflinks
PluriTest
Explorer
CGH
Tophat
Teratoscore
Tophat2
R
GATK HaplotypeCaller
Fluidigm

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