Assessment of estradiol-induced gene regulation and proliferation in an immortalized mouse immature Sertoli cell line

Life Sciences
Narender KumarPartha Roy

Abstract

The number of Sertoli cells during proliferative phase determines the fate of the germ cells in male reproductive system. A well-characterized cell line may help in better understanding of Sertoli cell biology. Hence, the present study assessed estradiol signaling in a mouse immature Sertoli cell line (MSC-1) as an alternative model in place of primary culture of Sertoli cells. In this study, we used MSC-1 cell line, derived from 10-day old mice. The cell cycle parameters were assessed, and the expression and regulation of Sertoli cell-specific secretory genes (ABP; androgen-binding protein) and tight junction genes (claudin-5, occludin, and vimentin) in response to estradiol was studied. The results obtained suggested the presence of both estrogen receptors (ERα and ERβ) in MSC-1 cells. In vitro scratch assay and cell-cycle analysis suggested the proliferative effects of estradiol in both time- and dose-dependent manner. The gene expression profiles of ABP, claudin-5, and occludin showed biphasic regulation at low and high doses of estradiol. Analysis of signaling pathways suggested the activation of extracellular signal-regulated kinase (ERK) pathway with significantly increased pERK/ERK ratio (p<0.05). The results also sugge...Continue Reading

References

Aug 1, 1992·Experimental Cell Research·M C HofmannJ L Millán
Dec 1, 1990·Endocrinology·B J DanzoH L Anthony
Jul 1, 1994·Biology of Reproduction·M P McGuinnessM D Griswold
Aug 4, 2004·Steroids·Tommaso SimonciniAndrea Riccardo Genazzani
Oct 9, 2004·The Journal of Clinical Endocrinology and Metabolism·Robin ZengGeorge I Gorodeski
Oct 13, 2007·Plant Methods·Erika Varkonyi-GasicRoger P Hellens
Jan 3, 2008·Cancer Cell·Sung-Liang YuPan-Chyr Yang
Aug 15, 2008·Genome Biology·Nicole CloonanSean M Grimmond
Jan 13, 2010·Cold Spring Harbor Perspectives in Biology·Andrea Oeckinghaus, Sankar Ghosh
Jun 18, 2010·Journal of Molecular Endocrinology·C BoisS Carreau
Jul 28, 2010·Nature Reviews. Genetics·Jacek KrolWitold Filipowicz
Nov 1, 2011·Pharmacological Reviews·C Yan Cheng, Dolores D Mruk
Feb 15, 2012·Spermatogenesis·Thaís Fg LucasCatarina S Porto
Jul 21, 2015·Molecular and Cellular Endocrinology·H M GaudetE J Filardo

❮ Previous
Next ❯

Citations

Mar 29, 2018·Reproductive Sciences·Asghar Beigi HarcheganiAlireza Shahriary
May 5, 2017·Journal of Animal Science and Biotechnology·Xiaoxu ChenWenxian Zeng

❮ Previous
Next ❯

Related Concepts

Related Feeds

Actin-binding Proteins

Actin-binding proteins are a component of the actin cytoskeleton that play essential roles in cellular functions such as regulation of actin polymerization, maintenance of cell polarity, gene expression regulation, cell motility and many more functions. Discover the latest research on actin-binding proteins here.