Assessment of estrogen receptors and apoptotic factors in cryopreserved human ovarian cortex

Systems Biology in Reproductive Medicine
Raffaella DepaloCatia Lippolis

Abstract

The aims of this study were: (i) to examine frozen-thawed ovarian tissues for features of follicular health and atresia by histology; (ii) to assess the expression of estrogen receptors alpha (ERalpha) and beta (ERbeta) by real-time PCR; (iii) to evaluate the Bax/Bcl-2 ratio, as an apoptotic index, in the ovarian tissues before and after cryopreservation. Ovarian cortical biopsies were obtained from 11 patients. The fragments were subdivided into two groups, fresh (control tissues) and cryopreserved tissues obtained by direct plunging into liquid nitrogen. Both tissue groups were subjected to a histological evaluation of the healthy and atretic follicles, immunohistochemical localization of the ER, and a real-time PCR (qPCR) to evaluate the expression of ER, Bax, Bcl-2 as well as beta-actin, as control gene. Damage was observed in 31% of primordial, 45% of primary, and 75% of secondary follicles in the cryopreserved tissue group. The qPCR analysis showed that the level of ERbeta was greater in fresh than cryopreserved tissues, whereas the ERalpha expression and Bax/Bcl-2 ratio were similar in both tissue groups. A significant inverse association was observed between ERalpha mRNA levels in the fresh tissue group and subjects' ag...Continue Reading

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Citations

Nov 21, 2013·Journal of Assisted Reproduction and Genetics·Fariba KhosraviFrederick W K Kan
Sep 25, 2012·Reproductive Biomedicine Online·Mojdeh SalehniaMonalill Lundqvist
Nov 15, 2011·Systems Biology in Reproductive Medicine·Amy E SiebertVirinder K Moudgil
Aug 14, 2010·Acta Biochimica Et Biophysica Sinica·Jie FuHaifeng Song

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