Assessment of GaN chips for culturing cerebellar granule neurons

Biomaterials
Tai-Horng Young, Chi-Ruei Chen

Abstract

In this work, the behaviors of cerebellar granule neurons prepared from 7-day-old Wistar rats on gallium nitride (GaN) were investigated. We believe that this is the first time that the GaN has been used as a substrate for neuron cultures to examine its effect on cell response in vitro. The GaN surface structure and its relationship with cells were examined by atomic force microscopy (AFM), metallography microscopy, scanning electron microscopy (SEM), lactate dehydrogenase (LDH) release and Western blot analysis. GaN is a so-called III-V compound semiconductor material with a wide bandgap and a relatively high bandgap voltage. Compared with silicon used for most neural chips, neurons seeded on GaN were able to form an extensive neuritic network and expressed very high levels of GAP-43 coincident with the neurite outgrowth. Therefore, the GaN structure may spatially mediate cellular response that can promote neuronal cell attachment, differentiation and neuritic growth. The favorable biocompatibility characteristics of GaN can be used to measure electric signals from networks of neuronal cells in culture to make it a possible candidate for use in a microelectrode array.

References

Jan 1, 1978·International Review of Cytology·F Grinnell
Dec 1, 1976·The Journal of Cell Biology·W L McKeehan, R G Ham
Feb 1, 1997·Trends in Neurosciences·L I Benowitz, A Routtenberg
Mar 5, 1999·Journal of Neuroscience Methods·S MartinoiaM Grattarola
Oct 18, 2000·Journal of Biomedical Materials Research·T H YoungJ P Hsu
Aug 30, 2001·Proceedings of the National Academy of Sciences of the United States of America·G Zeck, P Fromherz
Sep 28, 2002·Journal of Neuroscience Methods·Y W FanI-S Lee

❮ Previous
Next ❯

Citations

Oct 1, 2013·ACS Applied Materials & Interfaces·Jingying LiChen Wang
Jul 26, 2008·Nature Nanotechnology·Robert J MannixDonald E Ingber
Nov 10, 2011·Journal of Biomedical Science·Chia-Yi YuanYih-Jing Lee
Apr 11, 2012·EMBO Molecular Medicine·Francesc X GuixBart De Strooper
Sep 30, 2014·Colloids and Surfaces. B, Biointerfaces·Jingying LiChen Wang
Oct 25, 2011·Acta Biomaterialia·Scott A JewettAlbena Ivanisevic
Sep 20, 2008·Biomaterials·Cecilia E LinsmeierNils Danielsen
Jul 8, 2010·Angewandte Chemie·Jae-Hyun LeeJinwoo Cheon
Oct 10, 2013·Optics Express·H X Jiang, J Y Lin
Feb 19, 2009·Lab on a Chip·Jinyi WangShulin Chen
Nov 26, 2011·Chemical Society Reviews·David Richards, Albena Ivanisevic
Dec 6, 2014·Langmuir : the ACS Journal of Surfaces and Colloids·Nora G BergAlbena Ivanisevic
Jun 12, 2013·Langmuir : the ACS Journal of Surfaces and Colloids·Corey M FosterAlbena Ivanisevic
Dec 12, 2012·Langmuir : the ACS Journal of Surfaces and Colloids·Corey M FosterAlbena Ivanisevic
May 23, 2017·Nanoscale·Xiao Li, Xinyu Liu
Dec 21, 2017·Nature Communications·Chenlong ChenKui Xie
Apr 8, 2020·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Qingyun LiuZhiqiang Luo
Nov 19, 2020·Experimental Biology and Medicine·Isra MahaboobF Shahedipour-Sandvik
Dec 3, 2008·Analytical Chemistry·Chin-Pei ChenLi-Chyong Chen
May 29, 2021·Scientific Reports·Chaudhry Muhammad FurqanHoi-Sing Kwok
Aug 31, 2021·Materials Advances·Kyabeth M TorresRolando Oyola

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.