Assessment of genetic diversity among poplar (Populus deltoides Marsh.) clones from India using RAPD markers.

OSF Preprints
Rajeev KumarPrashant Kaushik

Abstract

Abstract Here we determined the genetic diversity among 19 Populus deltoides clones collected from forest nurseries of four districts of Haryana State. All of these were raised in the farm area of the Department of Forestry, and the leaf samples from the young plants of different clones of poplar were collected for molecular analysis. The extraction method (CTAB), DNA purification and, PCR reaction conditions were standardized to obtain genetic diversity. Out of the 30 RAPD markers used in this study, only 11 showed polymorphic pattern and showed a total of 94 alleles. Out of these, 59 were polymorphic, and 35 were monomorphic. An average number of alleles amplified was 8.54. The genetic similarity value calculated varied from 0.20 to 0.73 for 19 Poplar clones. The maximum similarity value (0.73) was observed between clone FRIAM 100 and W32, indicating FRIAM 100 and W32 to be most closely related genotypes. Despite low number of alleles that detected polymorphism, RAPD analysis indicated that there is high genetic diversity among Populus deltoides clones analyzed in this study. Since poplar is a crucial commercial agroforestry tree of Haryana state, this type of genetic characterization of the planting material is a pre-requisi...Continue Reading

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