Assessment of genetic fidelity in Rauvolfia serpentina plantlets grown from synthetic (encapsulated) seeds following in vitro storage at 4 °C.

Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry
Mohammad FaisalAhmad K Hegazy

Abstract

An efficient method was developed for plant regeneration and establishment from alginate encapsulated synthetic seeds of Rauvolfia serpentina. Synthetic seeds were produced using in vitro proliferated microshoots upon complexation of 3% sodium alginate prepared in Llyod and McCown woody plant medium (WPM) and 100 mM calcium chloride. Re-growth ability of encapsulated nodal segments was evaluated after storage at 4 °C for 0, 1, 2, 4, 6 and 8 weeks and compared with non-encapsulated buds. Effects of different media viz; Murashige and Skoog medium; Lloyd and McCown woody Plant medium, Gamborg’s B5 medium and Schenk and Hildebrandt medium was also investigated for conversion into plantlets. The maximum frequency of conversion into plantlets from encapsulated nodal segments stored at 4 °C for 4 weeks was achieved on woody plant medium supplement with 5.0 μM BA and 1.0 μM NAA. Rooting in plantlets was achieved in half-strength Murashige and Skoog liquid medium containing 0.5 μM indole-3-acetic acid (IAA) on filter paper bridges. Plantlets obtained from stored synseeds were hardened, established successfully ex vitro and were morphologically similar to each other as well as their mother plant. The genetic fidelity of Rauvolfia clones ...Continue Reading

References

Nov 25, 1990·Nucleic Acids Research·J G WilliamsS V Tingey
Apr 1, 1968·Experimental Cell Research·O L GamborgK Ojima
Oct 4, 2003·Environmental Science & Technology·C NoubactepB Merkel
Jan 1, 2009·Physiology and Molecular Biology of Plants : an International Journal of Functional Plant Biology·Hemant LataMahmoud A Elsohly

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Citations

Oct 6, 2012·Biotechnology Advances·Shiwali SharmaJaime A Teixeira da Silva
Aug 1, 2019·Applied Microbiology and Biotechnology·Eashan MukherjeeSomnath Bhattacharyya

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Methods Mentioned

BETA
electrophoresis
PCR

Software Mentioned

SPSS

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