Assessment of horizontal gene transfer in Lactic acid bacteria--a comparison of mating techniques with a view to optimising conjugation conditions

Journal of Microbiological Methods
Niamh ToomeyDeclan Bolton

Abstract

Plate, filter and broth mating techniques were assessed over a range of pHs using three Lactococcus lactis donor strains (one with an erythromycin resistance marker and two with tetracycline resistance markers, all located on transferable genetic elements) and one L. lactis recipient strain. Transconjugants were confirmed using antibiotic selection, E-tests to determine MICs, PCR assays to detect the corresponding marker genes, DNA fingerprinting by pulsed-field gel electrophoresis (PFGE), and Southern blotting. Horizontal gene transfer (HGT) rates varied (ranging from 1.6 x 10(-1) to 2.3 x 10(-8)). The general trend observed was plate > filter > broth, independent of pH. Our data suggests that standardisation of methodologies to be used to assess HGT, is warranted and would provide a meaningful assessment of the ability of commensal and other bacteria in different environments to transfer relevant markers.

References

Oct 6, 1999·Current Opinion in Microbiology·D A Rowe-Magnus, D Mazel
Mar 9, 2000·Trends in Microbiology·F de la Cruz, J Davies
Feb 24, 2001·Cellular and Molecular Life Sciences : CMLS·D Mazel, J Davies
Apr 20, 2001·Journal of Applied Microbiology·D WalshD A McDowell
Aug 22, 2001·Molecular and Cellular Probes·L K NgM Mulvey
Apr 5, 2002·Microbes and Infection·David G WhitePatrick F McDermott
Jun 10, 2003·Microbiology and Molecular Biology Reviews : MMBR·Elisabeth GrohmannManuel Espinosa
Aug 7, 2003·Proceedings of the National Academy of Sciences of the United States of America·C G KurlandOtto G Berg
Mar 10, 2004·Applied and Environmental Microbiology·Geert HuysJean Swings
Nov 18, 2005·International Journal of Food Microbiology·Shalini Mathur, Rameshwar Singh
Aug 5, 2008·International Journal of Food Microbiology·Joanna LampkowskaJacek Bardowski

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