Assessment of human MAPCs for stem cell transplantation and cardiac regeneration after myocardial infarction in SCID mice

Experimental Hematology
Ilias DimomeletisWolfgang M Franz

Abstract

Clinical studies suggest that transplantation of total bone marrow (BM) after myocardial infarction (MI) is feasible and potentially effective. However, focusing on a defined BM-derived stem cell type may enable a more specific and optimized treatment. Multilineage differentiation potential makes BM-derived multipotent adult progenitor cells (MAPCs) a promising stem cell pool for regenerative purposes. We analyzed the cardioregenerative potential of human MAPCs in a murine model of myocardial infarction. Human MAPCs were selected by negative depletion of CD45(+)/glycophorin(+) BM cells and plated on fibronectin-coated dishes. In vitro, stem cells were analyzed by reverse transcription polymerase chain reaction. In vivo, we transplanted human MAPCs (5 × 10(5)) by intramyocardial injection after MI in severe combined immunodeficient (SCID) beige mice. Six and 30 days after the surgical procedure, pressure-volume relationships were investigated in vivo. Heart tissues were analyzed immunohistochemically. Reverse transcription polymerase chain reaction experiments on early human MAPC passages evidenced an expression of Oct-4, a stem cell marker indicating pluripotency. In later passages, cardiac markers (Nkx2.5, GATA4, MLC-2v, MLC-2...Continue Reading

Citations

Jun 19, 2014·Clinical and Translational Medicine·Ariel A SimermanGregorio D Chazenbalk
Apr 22, 2014·Expert Opinion on Biological Therapy·Ariel A SimermanGregorio D Chazenbalk
Oct 7, 2011·Stem Cells International·Andrew WilberUma Lakshmipathy
Jun 20, 2015·Stem Cells International·Shuichiro Yamanaka, Takashi Yokoo
Feb 2, 2019·World Journal of Stem Cells·Luminita Labusca, Kaveh Mashayekhi
May 8, 2021·World Journal of Stem Cells·Nassem Ahamad, Brij B Singh

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