PMID: 7028212Dec 7, 1981Paper

Assessment of immunological properties of neurofilament triplet proteins

Brain Research
W W SchlaepferH L Wu

Abstract

The relationship between mammalian neurofilament triplet proteins was studied immunologically using rabbit and guinea pig antibodies to bovine neurofilament triplet proteins. Neurofilament proteins were separated by preparative electrophoresis, each protein being isolated and re-electrophoresed to enhance purification. Antisera to 68,000 (P68), 150,000 (P150) and 200,000 (P200) dalton neurofilament proteins showed greatest activity with the corresponding protein immunogen but also revealed cross-reactivity with the other two neurofilament proteins when assessed by the ELISA method. The same antigenic inoculum elicited variable cross-reactivity, more in the guinea pig than in the rabbit. Rabbit antisera to P68 was specific in that it did not cross-react with P150 or P200. Rabbit antisera to P150 and to P200 were rendered specific by absorption with P200 and P150, respectively. By electron microscopy, isolated neurofilaments became decorated with an uniform coat of antibodies when exposed to specific antisera for each of the neurofilament proteins. By indirect immunofluorescence, each antisera showed identical patterns of tissue localization, corresponding to the distribution of neurofilaments in peripheral nerve, spinal ganglia,...Continue Reading

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Citations

Jan 1, 1987·Cell and Tissue Research·K Meller
Jan 1, 1983·Acta Neuropathologica·J Q Trojanowski, V M Lee
Dec 1, 1983·Journal of the Neurological Sciences·I ElovaaraJ Palo
Feb 1, 1989·Journal of Neuroimmunology·J A BenjaminsM J Lefford
Jan 1, 1985·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·C Ayer-LelievreA Seiger
Jul 15, 1982·The New England Journal of Medicine·J Q TrojanowskiS Lee
Oct 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·V LeeW W Schlaepfer
May 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·M E GoldsteinN H Sternberger
May 1, 1981·Clinical and Experimental Pharmacology & Physiology·R A Dampney
May 15, 1987·American Journal of Ophthalmology·E PerentesV P Collins
Feb 1, 1986·Journal of Neuroimmunology·E P Eustis-TurfM J Lefford
Jan 1, 1991·Archives of Oral Biology·H Tsuzuki, H Kitamura
Feb 1, 1985·Journal of Neurochemistry·W W SchlaepferU J Zimmerman
Nov 1, 1983·Journal of Neurobiology·K Aoki, E J Muñoz-Martínez
Feb 1, 1983·Journal of Neurochemistry·B A BrownC A Marotta
Jan 1, 1987·Cell Motility and the Cytoskeleton·C E HartJ G Wood
Mar 1, 1984·The Journal of Comparative Neurology·A SeigerH Björklund
Mar 11, 1992·Clinical and Experimental Immunology·J Y ParkJ D Kim

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